Detection of multiple mycetoma pathogens using fungal metabarcoding analysis of soil DNA in an endemic area of Sudan.

Autor: Hashizume H; School of Tropical Medicine and Global Health, Nagasaki University, Nagasaki, Japan.; Department of Ecoepidemiology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan., Taga S; Department of Ecoepidemiology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan., Sakata MK; Graduate School of Human Development and Environment, Kobe University, Kobe, Japan., Taha MHM; Mycetoma Research Center, University of Khartoum, Khartoum, Sudan., Siddig EE; Mycetoma Research Center, University of Khartoum, Khartoum, Sudan., Minamoto T; Graduate School of Human Development and Environment, Kobe University, Kobe, Japan., Fahal AH; Mycetoma Research Center, University of Khartoum, Khartoum, Sudan., Kaneko S; School of Tropical Medicine and Global Health, Nagasaki University, Nagasaki, Japan.; Department of Ecoepidemiology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2022 Mar 11; Vol. 16 (3), pp. e0010274. Date of Electronic Publication: 2022 Mar 11 (Print Publication: 2022).
DOI: 10.1371/journal.pntd.0010274
Abstrakt: Mycetoma is a tropical disease caused by several fungi and bacteria present in the soil. Fungal mycetoma and eumycetoma are especially challenging to treat; therefore, prevention, early diagnosis, and early treatment are important, but it is also necessary to understand the geographic distribution of these pathogenic fungi. In this study, we used DNA metabarcoding methodology to identify fungal species from soil samples. Soil sampling was implemented at seven villages in an endemic area of Sennar State in Sudan in 2019, and ten sampling sites were selected in each village according to land-use conditions. In total, 70 soil samples were collected from ground surfaces, and DNA in the soil was extracted with a combined method of alkaline DNA extraction and a commercial soil DNA extraction kit. The region for universal primers was selected to be the ribosomal internal transcribed spacer one region for metabarcoding. After the second PCR for DNA library preparation, the amplicon-based DNA analysis was performed using next-generation sequencing with two sets of universal primers. A total of twelve mycetoma-causative fungal species were identified, including the prime agent, Madurella mycetomatis, and additional pathogens, Falciformispora senegalensis and Falciformispora tompkinsii, in 53 soil samples. This study demonstrated that soil DNA metabarcoding can elucidate the presence of multiple mycetoma-causative fungi, which may contribute to accurate diagnosis for patient treatment and geographical mapping.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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