| Autor: |
Fraczek M; Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland., Lewandowska A; Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland., Budzinska M; Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland., Kamieniczna M; Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland., Wojnar L; Clinic of Urology and Oncological Urology, Poznan University of Medical Sciences, 61-285 Poznan, Poland., Gill K; Department of Histology and Developmental Biology, Pomeranian Medical University in Szczecin, 71-210 Szczecin, Poland., Piasecka M; Department of Histology and Developmental Biology, Pomeranian Medical University in Szczecin, 71-210 Szczecin, Poland., Kups M; Department and Clinic Urology and Oncological Urology, Regional Specialist Hospital in Szczecin, 71-455 Szczecin, Poland.; The Fertility Partnership Vitrolive in Szczecin, 70-483 Szczecin, Poland., Havrylyuk A; Department of Clinical Immunology and Allergology, Danylo Halytskyy Lviv National Medical University, 79008 Lviv, Ukraine., Chopyak V; Department of Clinical Immunology and Allergology, Danylo Halytskyy Lviv National Medical University, 79008 Lviv, Ukraine., Nakonechnyy J; Department of Urology, Danylo Halytskyy Lviv National Medical University, 79010 Lviv, Ukraine., Nakonechnyy A; Department of Paediatric Surgery, Danylo Halytskyy Lviv National Medical University, 79059 Lviv, Ukraine., Kurpisz M; Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland. |
| Abstrakt: |
Responding to the need for the verification of some experimental animal studies showing the involvement of oxidative stress in germ cell damage in the heat-induced testis, we investigated the possibility of a direct relationship between seminal oxidative stress markers (total antioxidant capacity, catalase activity, superoxide dismutase activity, and malondialdehyde concentration) and ejaculated sperm chromatin/DNA integrity (DNA fragmentation and chromatin condensation abnormalities) in distinct groups of men exposed and not exposed to prolonged scrotal hyperthermia. A statistical increase in the proportion of sperm with DNA fragmentation was observed in all the studied subgroups compared to the fertile men. In turn, the groups subjected to heat stress as professional drivers or infertile men with varicocele presented greater disturbances in the oxidative stress scavenging system than men not exposed to genital heat stress. Based on the comparative analysis of the studied parameters, we can conclude that alterations in the seminal oxidative stress scavenging system are directly engaged in the pathogenesis of ejaculated sperm DNA damage regardless of the intensity of the impact of thermal insult. To the best of our knowledge, this study, for the first time, revealed the co-existence of oxidative stress and sperm DNA damage in the semen of professional drivers. |
