Optimization of Biocompatibility for a Hydrophilic Biological Molecule Encapsulation System.

Autor: Sanders AB; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Zangaro JT; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Webber NK; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Calhoun RP; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Richards EA; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Ricci SL; Department of Physics & Astronomy, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Work HM; Department of Physics & Astronomy, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Yang DD; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Casey KR; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Iovine JC; Department of Physics & Astronomy, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Baker G; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Douglas TV; Department of Physics & Astronomy, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Dutko SB; Department of Physics & Astronomy, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Fasano TJ; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Lofland SA; Department of Physics & Astronomy, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Rajan AA; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Vasile MA; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Carone BR; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA., Nucci NV; Department of Molecular and Cellular Biosciences, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA.; Department of Physics & Astronomy, Rowan University, 201 Mullica Hill Rd., Glassboro, NJ 08028, USA.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2022 Feb 27; Vol. 27 (5). Date of Electronic Publication: 2022 Feb 27.
DOI: 10.3390/molecules27051572
Abstrakt: Despite considerable advances in recent years, challenges in delivery and storage of biological drugs persist and may delay or prohibit their clinical application. Though nanoparticle-based approaches for small molecule drug encapsulation are mature, encapsulation of proteins remains problematic due to destabilization of the protein. Reverse micelles composed of decylmonoacyl glycerol (10MAG) and lauryldimethylamino-N-oxide (LDAO) in low-viscosity alkanes have been shown to preserve the structure and stability of a wide range of biological macromolecules. Here, we present a first step on developing this system as a future platform for storage and delivery of biological drugs by replacing the non-biocompatible alkane solvent with solvents currently used in small molecule delivery systems. Using a novel screening approach, we performed a comprehensive evaluation of the 10MAG/LDAO system using two preparation methods across seven biocompatible solvents with analysis of toxicity and encapsulation efficiency for each solvent. By using an inexpensive hydrophilic small molecule to test a wide range of conditions, we identify optimal solvent properties for further development. We validate the predictions from this screen with preliminary protein encapsulation tests. The insight provided lays the foundation for further development of this system toward long-term room-temperature storage of biologics or toward water-in-oil-in-water biologic delivery systems.
Databáze: MEDLINE
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