Methoxyeugenol Protects Against Lung Inflammation and Suppresses Neutrophil Extracellular Trap Formation in an LPS-Induced Acute Lung Injury Model.

Autor: Antunes GL; Laboratory of Cellular Biophysics and Inflammation, Pontifical Catholic University of Rio Grande Do Sul (PUCRS), 6690 Ipiranga Ave, Porto Alegre, RS, 90610-000, Brazil. gessica.antunes@edu.pucrs.br., Matzenbacher LS; Laboratory of Cellular Biophysics and Inflammation, Pontifical Catholic University of Rio Grande Do Sul (PUCRS), 6690 Ipiranga Ave, Porto Alegre, RS, 90610-000, Brazil., Costa BP; Laboratory of Cellular Biophysics and Inflammation, Pontifical Catholic University of Rio Grande Do Sul (PUCRS), 6690 Ipiranga Ave, Porto Alegre, RS, 90610-000, Brazil., de Sousa Basso B; Laboratory of Cellular Biophysics and Inflammation, Pontifical Catholic University of Rio Grande Do Sul (PUCRS), 6690 Ipiranga Ave, Porto Alegre, RS, 90610-000, Brazil., Levorse VGS; Laboratory of Cellular Biophysics and Inflammation, Pontifical Catholic University of Rio Grande Do Sul (PUCRS), 6690 Ipiranga Ave, Porto Alegre, RS, 90610-000, Brazil., Antunes KH; Laboratory of Clinical and Experimental Immunology, Pontifical Catholic University of Rio Grande Do Sul (PUCRS), Porto Alegre, Brazil., Costa-Ferro ZSM; Brain Institute of Rio Grande Do Sul (BraIns), Pontifical Catholic University of Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil., de Oliveira JR; Laboratory of Cellular Biophysics and Inflammation, Pontifical Catholic University of Rio Grande Do Sul (PUCRS), 6690 Ipiranga Ave, Porto Alegre, RS, 90610-000, Brazil.
Jazyk: angličtina
Zdroj: Inflammation [Inflammation] 2022 Aug; Vol. 45 (4), pp. 1534-1547. Date of Electronic Publication: 2022 Mar 10.
DOI: 10.1007/s10753-022-01639-8
Abstrakt: Acute lung injury (ALI) is a life-threatening acute inflammatory disease with high rates of morbidity and mortality worldwide. 4-Allyl-2,6-dimethoxyphenol (methoxyeugenol), a phenylpropanoid from a synthetic source, exhibits strong anti-inflammatory activity, but its effects on the inflammation of ALI have not yet been reported. In our study, the anti-inflammatory effects of methoxyeugenol were investigated on RAW 264.7 cells and a mice model of ALI. Our results showed that methoxyeugenol (7.5 and 30 µM) attenuated the proliferation and gene expression of interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells. In a mice model of ALI induced with LPS, methoxyeugenol exhibited a significant protective effect, based on influx reduction of macrophages and neutrophils into the lungs; reduction in release of the cytokines IL-6, TNF-α, and IL-10; and in reactive oxygen species (ROS) formation. We show that the anti-inflammatory effects of methoxyeugenol are associated with the suppression of the NFκB signaling pathway. Moreover, we demonstrated for the first time that a phenolic compound, from a synthetic source, protects against lung tissue inflammation and promotes a reduction of NET formation. These findings provided evidence for the use of methoxyeugenol as a new strategy to control inflammation in ALI disease.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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