Minocycline can reduce testicular apoptosis related to varicocele in male rats.

Autor: Shokoohi M; Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Clinical research development unit of Tabriz valiasr hospital, Tabriz University of Medical Sciences, Tabriz, Iran., Khaki A; Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Roudi Rasht Abadi A; Department of Animal Biology, Faculty of Biology, University of Kharazmi, Tehran, Iran., MohammadZadeh Boukani L; Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Hassanpour Khodaie S; Department of dentistry, Eastern Mediterranean University (EMU), Famagusta, Turkey., Kalarestaghi H; Department of Anatomical Sciences and Pathology, School of Medicine and Allied Medical Sciences, Ardabil University of Medical Sciences, Ardabil, Iran., Khaki AA; Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Department of Obstetrics and Gynecology, Universitätsklinikum Schleswig-Holstein Campus Lübeck, Lübeck, Germany., Moghimian M; Department of Physiology, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran., Niazkar HR; Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran., Shoorei H; Department of Anatomy & Cell Biology, Birjand University of Medical Sciences, Birjand, Iran.
Jazyk: angličtina
Zdroj: Andrologia [Andrologia] 2022 May; Vol. 54 (4), pp. e14375. Date of Electronic Publication: 2022 Mar 09.
DOI: 10.1111/and.14375
Abstrakt: The current research aimed to assess the impacts of Minocycline on varicocele-induced regulation of apoptotic-related genes and oxidative stress in the testis of adult Wistar rats. Thirty-two rats were divided into 4 groups: sham, varicocele (VcI), varicocele treated with Minocycline (VcI + Mno) for 56 days and healthy rats treated with minocycline (Mno). After 8 weeks, the oxidative stress markers levels in serum were investigated, afterwards, the level of Bax and Bcl-2 expression were assessed through 'immunocytochemistry' and RT-qPCR assays. Also, the rate of apoptosis was evaluated through the TUNEL method. Johnson's score, 'the width of epithelium' and 'seminiferous tubules diameter' were ameliorated in the VcI + Mno group in comparison with the Vcl group. Administration of Minocycline raised the 'Glutathione peroxidase' and 'Superoxide dismutase' levels in serum and declined the Malondialdehyde level in serum (p = 0.001). Furthermore, current study represented that minocycline reduced Bax and enhanced the expression of Bcl-2 gene and protein in comparison with the Vcl group (p < 0.05). In addition, Minocycline administration significantly declined the rate of apoptosis in germ cells (p < 0.05). Our study demonstrated that the administration of Minocycline could improve testicular injury in varicocele-induced rats by its antioxidant activity.
(© 2022 Wiley-VCH GmbH.)
Databáze: MEDLINE
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