Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins.

Autor:	Zhi Z; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.; Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia., Ma X; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.; University of Chinese Academy of Sciences, Beijing 100049, China., Zhou C; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China., Mechler A; Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia., Zhang S; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China., Liu P; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.; University of Chinese Academy of Sciences, Beijing 100049, China.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2022 Mar 03; Vol. 3 (1), pp. 101214. Date of Electronic Publication: 2022 Mar 03 (Print Publication: 2022).
DOI:	10.1016/j.xpro.2022.101214
Abstrakt:	Here, we present a protocol to construct artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins. We provide procedures to construct adiposomes and prepare recombinant lipid droplet-associated proteins. Then we describe approaches to measure the number density of perilipin 2 on natural lipid droplets, construct artificial lipid droplets, and determine the binding affinity of perilipin 2 on artificial lipid droplets. This protocol can be adapted to determine the binding properties of various lipid droplet-associated proteins. For complete details on the use and execution of this protocol, please refer to Ma et al. (2021). Competing Interests: P.L., X.M., Z.Z., and C.Z. have been authorized to hold a patent filed for the use of artificial LD and application thereof in evaluating interaction between LD-associated proteins and LDs. The interest has been fully disclosed to the Institute of Biophysics, Chinese Academy of Sciences. All other authors declare no competing interests. (© 2022 The Author(s).)
Databáze:	MEDLINE
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