A reduced SNP panel to trace gene flow across southern European wolf populations and detect hybridization with other Canis taxa.

Autor: Stronen AV; Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000, Ljubljana, Slovenia. astrid.stronen@gmail.com.; Department of Biotechnology and Life Sciences, Insubria University, via J. H. Dunant 3, 21100, Varese, Italy. astrid.stronen@gmail.com.; Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg, Denmark. astrid.stronen@gmail.com., Mattucci F; Unit for Conservation Genetics (BIO-CGE), Italian Institute for Environmental Protection and Research (ISPRA), Via Cà Fornacetta 9, 40064, Ozzano dell' Emilia, Bologna, Italy., Fabbri E; Unit for Conservation Genetics (BIO-CGE), Italian Institute for Environmental Protection and Research (ISPRA), Via Cà Fornacetta 9, 40064, Ozzano dell' Emilia, Bologna, Italy., Galaverni M; Science Unit, WWF Italia, Via Po 25c, 00198, Rome, Italy., Cocchiararo B; Wildlife Genetics Center, Senckenberg Research Institute and Natural History Museum Frankfurt, Clamecystrasse 12, 63571, Gelnhausen, Germany.; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Senckenberganlage 25, 60325, Frankfurt am Main, Germany., Nowak C; Wildlife Genetics Center, Senckenberg Research Institute and Natural History Museum Frankfurt, Clamecystrasse 12, 63571, Gelnhausen, Germany.; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Senckenberganlage 25, 60325, Frankfurt am Main, Germany., Godinho R; CIBIO/InBio - Centro de Investigaҫao em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal.; Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007, Porto, Portugal., Ruiz-González A; Department of Zoology and Animal Cell Biology, University of the Basque Country (UPV/EHU), Paseo de la Universidad, 7, 01006, Vitoria-Gasteiz, Spain., Kusak J; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000, Zagreb, Croatia., Skrbinšek T; Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000, Ljubljana, Slovenia., Randi E; Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg, Denmark., Vlasseva A; Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113, Sofia, Bulgaria., Mucci N; Unit for Conservation Genetics (BIO-CGE), Italian Institute for Environmental Protection and Research (ISPRA), Via Cà Fornacetta 9, 40064, Ozzano dell' Emilia, Bologna, Italy., Caniglia R; Unit for Conservation Genetics (BIO-CGE), Italian Institute for Environmental Protection and Research (ISPRA), Via Cà Fornacetta 9, 40064, Ozzano dell' Emilia, Bologna, Italy.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Mar 09; Vol. 12 (1), pp. 4195. Date of Electronic Publication: 2022 Mar 09.
DOI: 10.1038/s41598-022-08132-0
Abstrakt: Intra- and inter-specific gene flow are natural evolutionary processes. However, human-induced hybridization is a global conservation concern across taxa, and the development of discriminant genetic markers to differentiate among gene flow processes is essential. Wolves (Canis lupus) are affected by hybridization, particularly in southern Europe, where ongoing recolonization of historic ranges is augmenting gene flow among divergent populations. Our aim was to provide diagnostic canid markers focused on the long-divergent Iberian, Italian and Dinaric wolf populations, based on existing genomic resources. We used 158 canid samples to select a panel of highly informative single nucleotide polymorphisms (SNPs) to (i) distinguish wolves in the three regions from domestic dogs (C. l. familiaris) and golden jackals (C. aureus), and (ii) identify their first two hybrid generations. The resulting 192 SNPs correctly identified the five canid groups, all simulated first-generation (F1) hybrids (0.482 ≤ Q i  ≤ 0.512 between their respective parental groups) and all first backcross (BC1) individuals (0.723 ≤ Q i  ≤ 0.827 to parental groups). An assay design and test with invasive and non-invasive canid samples performed successfully for 178 SNPs. By separating natural population admixture from inter-specific hybridization, our reduced panel can help advance evolutionary research, monitoring, and timely conservation management.
(© 2022. The Author(s).)
Databáze: MEDLINE
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