The psychoactive effects of Bryophyllum pinnatum (Lam.) Oken leaves in young zebrafish.

Autor: Martins Fernandes Pereira K; Department of Obstetrics, Universidade Federal de São Paulo, São Paulo, SP, Brazil.; Department of Bioengineering and Therapeutic Sciences, Programs in Biological Sciences and Human Genetics, University of California, San Francisco, San Francisco, California, United States of America., Calheiros de Carvalho A; Department of Chemistry, Universidade Federal de São Paulo, Diadema, SP, Brazil., André Moura Veiga T; Department of Chemistry, Universidade Federal de São Paulo, Diadema, SP, Brazil., Melgoza A; Department of Bioengineering and Therapeutic Sciences, Programs in Biological Sciences and Human Genetics, University of California, San Francisco, San Francisco, California, United States of America.; Pharmaceutical Sciences and Pharmacogenomics Graduate Program, University of California, San Francisco, San Francisco, California, United States of America., Bonne Hernández R; Laboratory of Bioinorganic and Environmental Toxicology-LABITA, Department of Chemistry, Universidade Federal de São Paulo. Diadema. SP. Brazil., Dos Santos Grecco S; Triplet Biotechnology Solutions, São Paulo, Brazil., Uchiyama Nakamura M; Department of Obstetrics, Universidade Federal de São Paulo, São Paulo, SP, Brazil., Guo S; Department of Bioengineering and Therapeutic Sciences, Programs in Biological Sciences and Human Genetics, University of California, San Francisco, San Francisco, California, United States of America.; Pharmaceutical Sciences and Pharmacogenomics Graduate Program, University of California, San Francisco, San Francisco, California, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Mar 09; Vol. 17 (3), pp. e0264987. Date of Electronic Publication: 2022 Mar 09 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0264987
Abstrakt: Bryophyllum pinnatum (Lam.) Oken (BP) is a plant that is used worldwide to treat inflammation, infections, anxiety, restlessness, and sleep disorders. While it is known that BP leaves are rich in flavonoids, the extent of the beneficial and toxic effects of its crude extracts remains unclear. Although some neurobehavioral studies using leaf extracts have been conducted, none has examined the effects of water-extracted leaf samples. The zebrafish is a powerful animal model used to gain insights into the efficacy and toxicity profiles of this plant due to its high fecundity, external development, and ease of performing behavioral assays. In this study, we performed behavioral testing after acute exposure to different concentrations of aqueous extract from leaves of B. pinnatum (LABP) on larval zebrafish, investigating light/dark preference, thigmotaxis, and locomotor activity parameters under both normal and stressed conditions. LABP demonstrated dose-and time-dependent biphasic effects on larval behavior. Acute exposure (25 min) to 500 mg/L LABP resulted in decreased locomotor activity. Exposure to 300 mg/L LABP during the sleep cycle decreased dark avoidance and thigmotaxis while increasing swimming velocity. After sleep deprivation, the group treated with 100 mg/L LABP showed decreased dark avoidance and increased velocity. After a heating stressor, the 30 mg/L and 300 mg/L LABP-treated groups showed decreased dark avoidance. These results suggest both anxiolytic and psychoactive effects of LABP in a dose-dependent manner in a larval zebrafish model. These findings provide a better understanding of the mechanisms underlying relevant behavioral effects, consequently supporting the safe and effective use of LABP for the treatment of mood disorders.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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