CYTOKININ RESPONSE FACTOR 2 is involved in modulating the salt stress response.

Autor: Keshishian EA; Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA., Cliver BR; Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA., McLaughlin WF; Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA., Hallmark HT; Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA., Plačková L; Laboratory of Growth Regulators, Faculty of Science, Palacký University & Institute of Experimental Botany, The Czech Academy of Sciences, CZ-783 71, Olomouc, Czech Republic., Goertzen LR; Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA., Novák O; Laboratory of Growth Regulators, Faculty of Science, Palacký University & Institute of Experimental Botany, The Czech Academy of Sciences, CZ-783 71, Olomouc, Czech Republic., Cobine PA; Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA., Leisner CP; Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA., Rashotte AM; Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2022 May; Vol. 110 (4), pp. 1097-1110. Date of Electronic Publication: 2022 Mar 28.
DOI: 10.1111/tpj.15726
Abstrakt: Cytokinin has strong connections to development and a growing role in the abiotic stress response. Here we show that CYTOKININ RESPONSE FACTOR 2 (CRF2) is additionally involved in the salt (NaCl) stress response. CRF2 promoter-GUS expression indicates CRF2 involvement in the response to salt stress as well as the previously known cytokinin response. Interestingly, CRF2 mutant seedlings are quite similar to the wild type (WT) under non-stressed conditions yet have many distinct changes in response to salt stress. Cytokinin levels measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) that increased in the WT after salt stress are decreased in crf2, potentially from CRF2 regulation of cytokinin biosynthesis genes. Ion content measured by inductively coupled plasma optical emission spectrometry (ICP-OES) was increased in the WT for Na, K, Mn, Ca and Mg after salt stress, whereas the corresponding Ca and Mg increases are lacking in crf2. Many genes examined by RNA-seq analysis were altered transcriptionally by salt stress in both the WT and crf2, yet interestingly approximately one-third of salt-modified crf2 transcripts (2655) showed unique regulation. Different transcript profiles for salt stress in crf2 compared with the WT background was further supported through an examination of co-expressed genes by weighted gene correlation network analysis (WGCMA) and principal component analysis (PCA). Additionally, Gene Ontology (GO) enrichment terms found from salt-treated transcripts revealed most photosynthesis-related terms as only being affected in crf2, leading to an examination of chlorophyll levels and the efficiency of photosystem II (via the ratio of variable fluorescence to maximum fluorescence, F v /F m ) as well as physiology after salt treatment. Salt stress-treated crf2 plants had both reduced chlorophyll levels and lower F v /F m values compared with the WT, suggesting that CRF2 plays a role in the modulation of salt stress responses linked to photosynthesis.
(© 2022 Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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