Hypoglycemic effect on adult zebrafish (Danio rerio) of the 3β-6β-16β-trihydroxylup-20(29)-ene triterpene isolated from Combretum leprosum leaves in vivo and in silico approach.

Autor: Coutinho MR; Postgraduate Program in Biotechnology - PPGB-Renorbio, State University of Ceara, Fortaleza, Brazil., da Silva AW; Postgraduate Program in Biotechnology - PPGB-Renorbio, State University of Ceara, Fortaleza, Brazil., Ferreira MKA; Graduate Program in Natural Sciences, State University of Ceara, Fortaleza, Brazil., de Lima Rebouças E; Postgraduate Program in Biotechnology - PPGB-Renorbio, State University of Ceara, Fortaleza, Brazil., Mendes FRS; Department of Biological Chemistry, Regional University of Cariri, Crato, Brazil., Teixeira EH; Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil., Marinho EM; Department of Analytical Chemistry and Physical Chemistry, Federal University of Ceara, Fortaleza, Brazil., Marinho MM; Chemistry Course, State University of Vale do Acaraú, Sobral, Brazil., Marinho ES; Department of Chemistry, State University of Ceara, Limoeiro do Norte, Brazil., Teixeira AMR; Postgraduate Program in Biotechnology - PPGB-Renorbio, State University of Ceara, Fortaleza, Brazil.; Department of Biological Chemistry, Regional University of Cariri, Crato, Brazil., de Menezes JESA; Graduate Program in Natural Sciences, State University of Ceara, Fortaleza, Brazil., Dos Santos HS; Postgraduate Program in Biotechnology - PPGB-Renorbio, State University of Ceara, Fortaleza, Brazil.; Graduate Program in Natural Sciences, State University of Ceara, Fortaleza, Brazil.; Department of Biological Chemistry, Regional University of Cariri, Crato, Brazil.; Chemistry Course, State University of Vale do Acaraú, Sobral, Brazil.
Jazyk: angličtina
Zdroj: Fundamental & clinical pharmacology [Fundam Clin Pharmacol] 2022 Oct; Vol. 36 (5), pp. 818-826. Date of Electronic Publication: 2022 Mar 14.
DOI: 10.1111/fcp.12776
Abstrakt: Drugs used to manage type 2 diabetes mellitus cause adverse effects. Therefore, the search for new drugs as an alternative for the treatment of diabetes increases. The effect of triterpene 3β-6β-16β-trihydroxylup-20(29)-ene isolated from the leaves of C. leprosum (CLF-1) on sucrose-induced hyperglycemia in adult zebrafish (Danio rerio) was evaluated. Initially, adult zebrafish (n = 6/group) underwent hyperglycemia induction by sucrose at 83.25 mM/L for 7 days by immersion. The hyperglycemic groups were treated with CLF-1 (4, 20, and 40 mg/kg), metformin (200 mg/kg), and acarbose (300 mg/kg) for 4 days. The in silico interaction of CLF-1, metformin, and acarbose with the enzyme maltase-glucoamylase (CtMGAM) was investigated. CLF-1 reduced sucrose-induced hyperglycemia after 4 days of treatment, in addition to having better affinity energy with CtMGAM than metformin and acarbose. Thus, CLF-1 may be a new pharmacological alternative as a hypoglycemic agent for the treatment of diabetes.
(© 2022 Société Française de Pharmacologie et de Thérapeutique.)
Databáze: MEDLINE
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