Impact of silk hydrogel secondary structure on hydrogel formation, silk leaching and in vitro response.

Autor: Egan G; Department of Biomedical Engineering, Faculty of Engineering, University of Strathclyde, Glasgow, UK.; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK., Phuagkhaopong S; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK., Matthew SAL; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK., Connolly P; Department of Biomedical Engineering, Faculty of Engineering, University of Strathclyde, Glasgow, UK. patricia.connolly@strath.ac.uk., Seib FP; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, UK. philipp.seib@strath.ac.uk.; EPSRC Future Manufacturing Research Hub for Continuous Manufacturing and Advanced Crystallisation (CMAC), University of Strathclyde, Technology and Innovation Centre, 99 George Street, Glasgow, G1 1RD, UK. philipp.seib@strath.ac.uk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Mar 08; Vol. 12 (1), pp. 3729. Date of Electronic Publication: 2022 Mar 08.
DOI: 10.1038/s41598-022-07437-4
Abstrakt: Silk can be processed into a broad spectrum of material formats and is explored for a wide range of medical applications, including hydrogels for wound care. The current paradigm is that solution-stable silk fibroin in the hydrogels is responsible for their therapeutic response in wound healing. Here, we generated physically cross-linked silk fibroin hydrogels with tuned secondary structure and examined their ability to influence their biological response by leaching silk fibroin. Significantly more silk fibroin leached from hydrogels with an amorphous silk fibroin structure than with a beta sheet-rich silk fibroin structure, although all hydrogels leached silk fibroin. The leached silk was biologically active, as it induced vitro chemokinesis and faster scratch assay wound healing by activating receptor tyrosine kinases. Overall, these effects are desirable for wound management and show the promise of silk fibroin and hydrogel leaching in the wider healthcare setting.
(© 2022. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje