Ticks of domestic animals in Lesotho: Morphological and molecular characterization.

Autor: Mahlobo-Shwabede SIC; Discipline of Genetics, School of Life Science, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Durban 4000, South Africa., Zishiri OT; Discipline of Genetics, School of Life Science, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Durban 4000, South Africa. Electronic address: Zishiri@ukzn.ac.za., Thekisoe OMM; Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2531, South Africa., Bakkes D; Gertrud Theiler Tick Museum, Agricultural Research Council-Onderstepoort Veterinary Research, Pretoria, South Africa., Bohloa L; Department of Livestock Services, Ministry of Agriculture and Food Security, Maseru 100, Lesotho., Molomo M; Department of Livestock Services, Ministry of Agriculture and Food Security, Maseru 100, Lesotho., Makalo MJR; Department of Livestock Services, Ministry of Agriculture and Food Security, Maseru 100, Lesotho., Mahloane GR; Department of Livestock Services, Ministry of Agriculture and Food Security, Maseru 100, Lesotho., Mtshali MS; University of Limpopo, School of Molecular and Life Sciences, Private Bag X1106, Sovenga 0727, South Africa.
Jazyk: angličtina
Zdroj: Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2022 Apr; Vol. 29, pp. 100691. Date of Electronic Publication: 2022 Jan 20.
DOI: 10.1016/j.vprsr.2022.100691
Abstrakt: A total of 3311 tick specimens were randomly collected from domestic animals including cattle, sheep, goats, horses, donkeys, and dogs from Lesotho districts namely, Berea, Butha-Buthe, Leribe, Mafeteng, Maseru, Mohale's Hoek, Mokhotlong, Qacha's Nek, Quthing and Thaba Tseka. Tick species were identified morphologically and verified by amplification and sequencing of the CO1 and 18S rRNA genes. Nine species were identified under different genera namely, Haemaphysalis elliptica 0.1% (n = 2), Hyalomma rufipes 2.6% (n = 87), Hy. truncatum 1.2% (n = 41), Otobius megnini 13.6% (n = 451), Rhipicephalus appendiculatus 0.1% (n = 3), Rhipicephalus decoloratus 9.3% (n = 308), Rhipicephalus evertsi evertsi 65.1% (n = 2156), Rhipicephalus glabroscutatum 1.3% (n = 43) and Rhipicephalus microplus 6.6% (n = 220). There was a significant difference at p = 6.2E-06 (ꭓ 2  = 1.923, df = 7) in the distribution of tick species and their abundance p = 0.04 (ꭓ 2  = 1.923, df = 7) from each population. The CO1 and 18S rRNA sequences matched the morphological determinations on the NCBI database and clustered with relevant species on the phylogenetic tree. Genetic analysis of CO1 and 18S rRNA provided very strong support for monophyly of the Rhipicephalinae and Ornithodorinae complexes. Both CO1 and 18S rRNA are useful genetic markers for the specific and generic characterization of tick species in Lesotho and elsewhere. This is the first scientific publication of tick species occurring in Lesotho.
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Databáze: MEDLINE
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