Effects of Se(IV) or Se(VI) enrichment on proteins and protein-bound Se distribution and Se bioaccessibility in oyster mushrooms.

Autor: de Oliveira AP; University of Cincinnati, Cincinnati, OH, United States; Universidade Federal de São Paulo, Diadema, Sao Paulo, Brazil., Naozuka J; Universidade Federal de São Paulo, Diadema, Sao Paulo, Brazil. Electronic address: j.naozuka@unifesp.br., Landero-Figueroa JA; University of Cincinnati, Cincinnati, OH, United States.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2022 Jul 30; Vol. 383, pp. 132582. Date of Electronic Publication: 2022 Feb 26.
DOI: 10.1016/j.foodchem.2022.132582
Abstrakt: A successful mushroom enrichment process must produce foods that have compounds potentially absorbed by the human body. In this study, Pleurotus ostreatus and Pleurotus djamor mushrooms were grown on organic substrate supplemented with different Se(IV) and Se(VI) concentrations, and evaluated in the following features: Fruiting bodies morphology; Se uptake and accumulation; Distribution of proteins and protein-bound Se; Se species identification on enzymatic extracts; Se bioaccessibility; and Distribution of bioaccessible protein-bound Se. Pleurotus djamor grown on Se(IV)-supplemented substrate showed the greatest potential to uptake and accumulate Se. For Se species screening, selenomethionine was identified in white oyster mushroom, while selenomethionine, selenocystine, and Se-methylselenocysteine in pink oyster mushrooms. In soluble fractions from in vitro gastrointestinal digestion assays, Se showed high bioaccessibility (>94%). Lastly, bioaccessible Se species were found to be mainly associated to LMW (<17 kDa) in Pleurotus ostreatus (74%) and Pleurotus djamor (68%) grown on Se(IV)-supplemented substrates.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: