Natronosporangium hydrolyticum gen. nov., sp. nov., a haloalkaliphilic polyhydrolytic actinobacterium from a soda solonchak soil in Central Asia.

Autor: Sorokin DY; Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia; Department of Biotechnology, TU Delft, the Netherlands. Electronic address: soroc@inmi.ru., Elcheninov AG; Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Khijniak TV; Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Zaharycheva AP; Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Boueva OV; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Puschino, Moscow Region, Russia., Ariskina EV; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Puschino, Moscow Region, Russia., Bunk B; Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Germany., Spröer C; Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Germany., Evtushenko LI; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Puschino, Moscow Region, Russia., Kublanov IV; Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Hahnke RL; Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Germany.
Jazyk: angličtina
Zdroj: Systematic and applied microbiology [Syst Appl Microbiol] 2022 May; Vol. 45 (3), pp. 126307. Date of Electronic Publication: 2022 Feb 24.
DOI: 10.1016/j.syapm.2022.126307
Abstrakt: During a cultural diversity survey on hydrolytic bacteria in saline alkaline soils, a hydrolytic actinobacterium strain ACPA39 T was enriched and isolated in pure culture from a soda solonchak soil in southwestern Siberia. It forms a substrate mycelium with rod-shaped sporangia containing 1-3 exospores. The isolate is obligately alkaliphilic, growing at pH 7.5-10.3 (optimum at 8.5-9.0) and moderately halophilic, tolerating up to 3 M total Na + in the form of sodium carbonates. It is an obligately aerobic, organoheteroterophic, saccharolytic bacterium, utilizing various sugars and alpha/beta-glucans as growth substrates. According to the 16S rRNA gene-based phylogenetic analysis, strain ACPA39 T forms a distinct branch within the family Micromonosporaceae, with the sequence identities below 94.5% with type strains of other genera. This is confirmed by phylogenomic analysis based on the 120 conserved single copy protein-based markers and genomic indexes (ANI, AAI). The cell-wall of ACPA39 T contained meso-DAP, glycine, glutamic acid and alanine in a equimolar ratio, characteristic of the peptidoglycan type A1γ'. The whole-cell sugars include galactose and xylose. The major menaquinone is MK-10(H 4 ). The identified polar lipids consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The polar lipid fatty acids were dominated by anteiso-C 17:0 , iso-C 16:0 , iso-C 17:0 , 10 Me-C 18:0 and C 18:1 ω9. Based on the distinct phylogeny, the chemotaxonomy features and unique phenotypic properties, strain ACPA39 T (DSM 106523 T  = VKM 2772 T ) is classified into a new genus and species in the family Micromonosporaceae for which the name Natronosporangium hydrolitycum gen. nov., sp. nov. is proposed.
(Copyright © 2022 The Author(s). Published by Elsevier GmbH.. All rights reserved.)
Databáze: MEDLINE
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