Evolving Patterns of Hyperfluorescent Fundus Autofluorescence Accompany Retinal Atrophy in the Rat and Mimic Atrophic Age-Related Macular Degeneration.

Autor:	Pankova N; Department of Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, Canada.; Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada.; Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada., Baek DSH; Department of Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, Canada.; Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada.; Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada., Zhao X; Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada., Wang H; Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada., Reyad MM; Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada., Liang H; Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada., Joshi R; Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada., Boyd SR; Department of Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, Canada.; Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada.; Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
Jazyk:	angličtina
Zdroj:	Translational vision science & technology [Transl Vis Sci Technol] 2022 Mar 02; Vol. 11 (3), pp. 3.
DOI:	10.1167/tvst.11.3.3
Abstrakt:	Purpose: Complex two-dimensional (2D) patterns of hyperfluorescent short-wave fundus autofluorescence (FAF) at the border of geographic atrophy (GA) can predict its expansion in patients with late non-exudative "dry" AMD. However, preclinical models do not phenocopy this important feature of disease. We sought to describe the spatiotemporal changes in hyperfluorescent FAF patterns that occur following acute oxidative stress, potentially in association with GA expansion. Methods: Sprague Dawley rats (n = 54) received systemic sodium iodate (25-45 mg/kg, n = 90 eyes) or saline (n = 18 eyes) and underwent serial full fundus imaging by confocal scanning laser ophthalmoscopy, including blue FAF and delayed near-infrared analysis. Composite images of the fundus were assembled, and the 2D patterns were described qualitatively and quantitatively. A subset of eyes underwent tissue analysis, and four underwent optical coherence tomography (OCT) imaging. Results: Reproducibly changing, complex patterns of hyperfluorescent FAF emerge at the borders of toxin-induced damage; however, in the absence of GA expansion, they percolate inward within the region of retinal pigment epithelium loss, evolving, maturing, and senescing in situ over time. Unexpectedly, the late FAF patterns most closely resemble the diffuse tricking form of clinical disease. A five-stage classification system is presented. Conclusions: Longitudinal, full-fundus imaging of outer retinal atrophy in the rat eye identifies evolving, complex patterns of hyperfluorescent FAF that phenocopy aspects of disease. Translational Relevance: This work provides a novel tool to assess hyperfluorescent FAF in association with progressive retinal atrophy, a therapeutic target in late AMD.
Databáze:	MEDLINE
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