Mitochondrial Targeting of Amyloid-β Protein Precursor Intracellular Domain Induces Hippocampal Cell Death via a Mechanism Distinct from Amyloid-β.

Autor:	Sandberg AA; Department of Biological Sciences, University of Denver, Denver, CO, USA., Manning E; Department of Biological Sciences, University of Denver, Denver, CO, USA., Wilkins HM; Department of Biological Sciences, University of Denver, Denver, CO, USA.; Department of Neurology, University of Kansas Alzheimer's Disease Center, University of Kansas Medical Center, Kansas City, KS, USA., Mazzarino R; Department of Biological Sciences, University of Denver, Denver, CO, USA., Minckley T; Department of Biological Sciences, University of Denver, Denver, CO, USA., Swerdlow RH; Department of Neurology, University of Kansas Alzheimer's Disease Center, University of Kansas Medical Center, Kansas City, KS, USA., Patterson D; Knoebel Institute for Healthy Aging and Eleanor Roosevelt Institute, University of Denver, Denver, CO, USA., Qin Y; Department of Biological Sciences, University of Denver, Denver, CO, USA., Linseman DA; Department of Biological Sciences, University of Denver, Denver, CO, USA.; Knoebel Institute for Healthy Aging and Eleanor Roosevelt Institute, University of Denver, Denver, CO, USA.
Jazyk:	angličtina
Zdroj:	Journal of Alzheimer's disease : JAD [J Alzheimers Dis] 2022; Vol. 86 (4), pp. 1727-1744.
DOI:	10.3233/JAD-215108
Abstrakt:	Background: Amyloid-β (Aβ) is a principal cleavage product of amyloid-β protein precursor (AβPP) and is widely recognized as a key pathogenic player in Alzheimer's disease (AD). Yet, there is increasing evidence of a neurotoxic role for the AβPP intracellular domain (AICD) which has been proposed to occur through its nuclear function. Intriguingly, there is a γ-secretase resident at the mitochondria which could produce AICD locally. Objective: We examined the potential of AICD to induce neuronal apoptosis when targeted specifically to the mitochondria and compared its mechanism of neurotoxicity to that of Aβ. Methods: We utilized transient transfection of HT22 neuronal cells with bicistronic plasmids coding for DsRed and either empty vector (Ires), Aβ, AICD59, or mitochondrial-targeted AICD (mitoAICD) in combination with various inhibitors of pathways involved in apoptosis. Results: AICD induced significant neuronal apoptosis only when targeted to the mitochondria. Apoptosis required functional mitochondria as neither Aβ nor mitoAICD induced significant toxicity in cells devoid of mitochondrial DNA. Both glutathione and a Bax inhibitor protected HT22 cells from either peptide. However, inhibition of the mitochondrial permeability transition pore only protected from Aβ, while pan-caspase inhibitors uniquely rescued cells from mitoAICD. Conclusion: Our results show that AICD displays a novel neurotoxic function when targeted to mitochondria. Moreover, mitoAICD induces apoptosis via a mechanism that is distinct from that of Aβ. These findings suggest that AICD produced locally at mitochondria via organelle-specific γ-secretase could act in a synergistic manner with Aβ to cause mitochondrial dysfunction and neuronal death in AD.
Databáze:	MEDLINE
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