Urinary hsv2-miR-H9 to hsa-miR-3659 ratio is an effective marker for discriminating prostate cancer from benign prostate hyperplasia in patients within the prostate-specific antigen grey zone.

Autor:	Kang HW; Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea.; Department of Urology, Chungbuk National University Hospital, Cheongju, Korea., Byun YJ; Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea., Moon SM; Department of Urology, Chungbuk National University Hospital, Cheongju, Korea., Kim K; Department of Urology, Chungbuk National University Hospital, Cheongju, Korea., Piao XM; Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea., Zheng CM; Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea., Moon SK; Department of Food Science and Technology, Chung-Ang University, Ansung, Korea., Choi YH; Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan, Korea., Kim WT; Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea.; Department of Urology, Chungbuk National University Hospital, Cheongju, Korea., Kim YJ; Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea.; Department of Urology, Chungbuk National University Hospital, Cheongju, Korea., Lee SC; Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea.; Department of Urology, Chungbuk National University Hospital, Cheongju, Korea., Yun SJ; Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea.; Department of Urology, Chungbuk National University Hospital, Cheongju, Korea. sjyun@chungbuk.ac.kr., Kim WJ; Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea.; Institute of Urotech, Cheongju, Korea. wjkim@chungbuk.ac.kr.
Jazyk:	angličtina
Zdroj:	Investigative and clinical urology [Investig Clin Urol] 2022 Mar; Vol. 63 (2), pp. 238-244.
DOI:	10.4111/icu.20210493
Abstrakt:	Purpose: Tumor microRNAs (miRNAs) are released to biofluids directly or indirectly. Although urinary miRNAs are promising non-invasive biomarkers for the diagnosis of prostate cancer (PCa), their clinical application is challenging for technical reasons. We examined the efficacy of urinary hsv2-miR-H9 to hsa-miR-3659 ratio as a non-invasive diagnostic biomarker of PCa. Materials and Methods: The expression of urinary miRNAs was quantified by real-time PCR in 116 samples from 53 patients with benign prostatic hyperplasia (BPH) and 63 patients with PCa. The miRNA expression ratio was calculated using an upregulated miRNA (hsv2-miR-H9) as the numerator and a downregulated miRNA (hsa-miR-3659) as the denominator. Results: The urinary miR-H9 to miR-3659 ratio was significantly higher in PCa than in BPH controls (p<0.001). The diagnostic accuracy of the urinary miRNA expression ratio was comparable with that of prostate-specific antigen (PSA) (receiver operating characteristic [ROC] curve comparison, p=0.287). The area under the curve for urinary miRNA expression ratio was 0.862 and that for PSA was 0.642 in the "PSA gray zone" (3-10 ng/mL) (ROC curve comparison, p=0.034). The use of the urinary miRNA expression ratio would have prevented 70.6% of unnecessary prostate biopsies; however, 28.6% of PCa cases could be missed in patients within the PSA gray zone. Conclusions: The expression ratio of urinary miR-H9 to miR-3659 could be a relevant non-invasive biomarker for PCa diagnosis, particularly for patients within the PSA gray zone. Competing Interests: The authors have nothing to disclose. (© The Korean Urological Association.)
Databáze:	MEDLINE
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