Comprehensive analysis of phenolics compounds in citrus fruits peels by UPLC-PDA and UPLC-Q/TOF MS using a fused-core column.

Autor:	Sanches VL; Multidisciplinary Laboratory of Food and Health (LabMAS), School of Applied Sciences (FCA), University of Campinas (UNICAMP), Rua Pedro Zaccaria, 1300, 12383-250 Limeira, SP, Brazil., Cunha TA; Department of Clinical Analysis, Toxicology and Food Science, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, Ribeirão Preto, SP 14040-903, Brazil., Viganó J; Multidisciplinary Laboratory of Food and Health (LabMAS), School of Applied Sciences (FCA), University of Campinas (UNICAMP), Rua Pedro Zaccaria, 1300, 12383-250 Limeira, SP, Brazil., de Souza Mesquita LM; Multidisciplinary Laboratory of Food and Health (LabMAS), School of Applied Sciences (FCA), University of Campinas (UNICAMP), Rua Pedro Zaccaria, 1300, 12383-250 Limeira, SP, Brazil., Faccioli LH; Department of Clinical Analysis, Toxicology and Food Science, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, Ribeirão Preto, SP 14040-903, Brazil., Breitkreitz MC; Laboratory of Pharmaceutial Research and Chemometrics (LabFarQui), Institute of Chemistry, University of Campinas (UNICAMP), Rua Josué de Castro s/n, 13083-970, Campinas, São Paulo, Brazil., Rostagno MA; Multidisciplinary Laboratory of Food and Health (LabMAS), School of Applied Sciences (FCA), University of Campinas (UNICAMP), Rua Pedro Zaccaria, 1300, 12383-250 Limeira, SP, Brazil.
Jazyk:	angličtina
Zdroj:	Food chemistry: X [Food Chem X] 2022 Feb 22; Vol. 14, pp. 100262. Date of Electronic Publication: 2022 Feb 22 (Print Publication: 2022).
DOI:	10.1016/j.fochx.2022.100262
Abstrakt:	In this work, a method based on ultra-high-performance liquid chromatography with a photodiode array detector (UPLC-PDA) was developed to comprehensively analyze phenolic compounds in peels of lime ( Citrus × latifolia ), lemon ( Citrus limon ), and rangpur lime ( Citrus × limonia ). The reverse-phase separation was achieved with a C18 fused-core column packed with the smallest particles commercially available (1.3 um). The method was successfully coupled with high-resolution mass spectrometry (HRMS), allowing the detection of 24 phenolic compounds and five limonoids in several other citrus peels species: key lime, orange and sweet orange, tangerine, and tangerine ponkan, proving the suitability for comprehensive analysis in citrus peel matrices. Additionally, the developed method was validated according to the Food and drug administration (FDA) and National Institute of Metrology Quality and Technology (INMETRO) criteria, demonstrating specificity, linearity, accuracy, and precision according to these guidelines. System suitability parameters such as resolution, tailoring, plate count were also verified. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2022 The Authors.)
Databáze:	MEDLINE
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