Diversity of Sclerotium rolfsii antagonist fungi isolated from soils of the rhizosphere of tomato crops and identification of some antifungal compounds.

Autor:	Coulibaly AE; Laboratoire Biologie et Santé, UFR Biosciences, Université Félix Houphouët-Boigny d'Abidjan (UFHB), 22 BP 582, Abidjan 22, Côte d'Ivoire., Pakora GA; Laboratoire Biologie et Santé, UFR Biosciences, Université Félix Houphouët-Boigny d'Abidjan (UFHB), 22 BP 582, Abidjan 22, Côte d'Ivoire.; Laboratoire Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum National d'Histoire naturelle, CNRS, CP54, 57 Rue Cuvier, 75005, Paris, France., Ako ABA; Departement de Parasitologie and Mycologie, Institut Pasteur de Côte d'Ivoire, Côte d'Ivoire., Amari GELD; Laboratoire de Physiologie Végétale, UFR Biosciences, Université Félix Houphouët-Boigny d'Abidjan (UFHB), 22 BP 582, Abidjan 22, Cote d'Ivoire., N'Guessan CA; Departement Biologie Vegetale, Université Péléforo Gon Coulibaly, Korhogo, Côte d'Ivoire., Kouabenan A; Laboratoire de Phytopathologie et de Biologie Végétale, Institut National Polytechnique Félix Houphouët-Boigny (INP-HB), B.P. 1313, Yamoussoukro, Côte d'Ivoire., Kone D; Laboratoire de Physiologie Végétale, UFR Biosciences, Université Félix Houphouët-Boigny d'Abidjan (UFHB), 22 BP 582, Abidjan 22, Cote d'Ivoire., Djaman JA; Laboratoire Biologie et Santé, UFR Biosciences, Université Félix Houphouët-Boigny d'Abidjan (UFHB), 22 BP 582, Abidjan 22, Côte d'Ivoire.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2022 Feb 11; Vol. 8 (2), pp. e08943. Date of Electronic Publication: 2022 Feb 11 (Print Publication: 2022).
DOI:	10.1016/j.heliyon.2022.e08943
Abstrakt:	Sclerotium rolfsii Sacc. the causative agent of white rot is one of the destructive pathogens of nightshade crops. In Côte d'Ivoire, this fungal pathogen constitutes a major constraint for the cultivation of tomato ( Solanum lycopersicum ) with 41.01% crop losses in humid forest areas. Controlling this fungus with synthetic chemicals can be effective, but harmful to human health and the environment. The use of biological control agents could be an alternative approach to control S. rolfsii. In this perspective, the objective of this work was to select fungi from the rhizosphere of tomato crops capable of inhibiting the growth of S. rolfsii. To do this, 153 fungi were isolated from the rhizosphere and from direct confrontation tests 10 fungi whose antagonistic power of S. rolfsii varied between 27 and 60% were selected. Molecular identification (ITS) of these antagonist fungi revealed that the isolates belonged to the genera Talaromyces sp. (n = 4), Trichoderma sp. (n = 3), Penicillium sp. (n = 2) and Clonostachys sp. (n = 1). Among these fungi, Talaromyces purpureogenus and Talaromyces assiutensis were able to diffuse compounds in agar capable of inhibiting the growth of S. rolfsii. The chemical study of these 2 fungi made it possible to identify mitorubrin and mitorubrinol produced by T. purpureogenus and spiculisporic acid produced by T. assiutensis. Mitorubrin and mitorubrinol had inhibitory activities of 100 and 70% at 10 mg/mL, respectively, whereas spiculisporic acid showed moderate inhibition of 38 at 20 mg/mL of the growth of S. rolfsii; however, its abundant production by the fungus could be an advantage in the control of this phytopathogen. Isolated from the same biotope as S. rolfsii, T. purpureogenus and T. assiutensis represent favorable candidates for the biological control against S. rolfsii. Competing Interests: The authors declare no conflict of interest. (© 2022 The Author(s).)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu