Chromosome-scale Echinococcus granulosus (genotype G1) genome reveals the Eg95 gene family and conservation of the EG95-vaccine molecule.

Autor: Korhonen PK; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Kinkar L; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Young ND; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Cai H; BGI-Shenzhen, Shenzhen, 518083, China.; Shenzhen Key Laboratory of Unknown Pathogen Identification, BGI-Shenzhen, Shenzhen, 518083, China., Lightowlers MW; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Gauci C; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Jabbar A; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Chang BCH; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Wang T; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Hofmann A; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Koehler AV; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Li J; BGI-Shenzhen, Shenzhen, 518083, China.; Shenzhen Key Laboratory of Unknown Pathogen Identification, BGI-Shenzhen, Shenzhen, 518083, China., Li J; BGI-Shenzhen, Shenzhen, 518083, China.; Shenzhen Key Laboratory of Unknown Pathogen Identification, BGI-Shenzhen, Shenzhen, 518083, China., Wang D; BGI-Shenzhen, Shenzhen, 518083, China.; Shenzhen Key Laboratory of Unknown Pathogen Identification, BGI-Shenzhen, Shenzhen, 518083, China., Yin J; BGI-Shenzhen, Shenzhen, 518083, China.; Shenzhen Key Laboratory of Unknown Pathogen Identification, BGI-Shenzhen, Shenzhen, 518083, China., Yang H; BGI-Shenzhen, Shenzhen, 518083, China.; Shenzhen Key Laboratory of Unknown Pathogen Identification, BGI-Shenzhen, Shenzhen, 518083, China., Jenkins DJ; School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW, 2678, Australia., Saarma U; Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, J. Liivi 2, Tartu, 50409, Estonia., Laurimäe T; Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, J. Liivi 2, Tartu, 50409, Estonia., Rostami-Nejad M; Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Irshadullah M; Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, 202002, India., Mirhendi H; Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Sharbatkhori M; Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran., Ponce-Gordo F; Department of Parasitology, Faculty of Pharmacy, Complutense University, Plaza Ramón y Cajal s/n, 28040, Madrid, Spain., Simsek S; Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Turkey., Casulli A; World Health Organization Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis, European Union Reference Laboratory for Parasites (EURLP), Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy., Zait H; Parasitology and Mycology Department, Mustapha University Hospital, 16000, Algiers, Algeria., Atoyan H; Yerevan State University, Department of Zoology, Alex Manoogian, Yerevan, 0025, Armenia., de la Rue ML; Universidade Federal de Santa Maria, Departamento de Microbiologia e Parasitologia, Santa Maria, RS, Brazil., Romig T; Institute of Zoology, Parasitology Unit, University of Hohenheim, 70599, Stuttgart, Germany., Wassermann M; Institute of Zoology, Parasitology Unit, University of Hohenheim, 70599, Stuttgart, Germany., Aghayan SA; Chair of Zoology, Yerevan State University, 1 Alex Manoogian, Yerevan, 0025, Armenia., Gevorgyan H; Molecular Parasitology Research Group, Scientific Center - Zoology and Hydroecology, 7P. Sevak str, Yerevan, 0014, Armenia., Yang B; BGI Australia, Oceania, BGI Group, CBCRB Building, Herston Road, Herston, QLD, 4006, Australia., Gasser RB; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia. robinbg@unimelb.edu.au.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2022 Mar 03; Vol. 5 (1), pp. 199. Date of Electronic Publication: 2022 Mar 03.
DOI: 10.1038/s42003-022-03125-1
Abstrakt: Cystic echinococcosis is a socioeconomically important parasitic disease caused by the larval stage of the canid tapeworm Echinococcus granulosus, afflicting millions of humans and animals worldwide. The development of a vaccine (called EG95) has been the most notable translational advance in the fight against this disease in animals. However, almost nothing is known about the genomic organisation/location of the family of genes encoding EG95 and related molecules, the extent of their conservation or their functions. The lack of a complete reference genome for E. granulosus genotype G1 has been a major obstacle to addressing these areas. Here, we assembled a chromosomal-scale genome for this genotype by scaffolding to a high quality genome for the congener E. multilocularis, localised Eg95 gene family members in this genome, and evaluated the conservation of the EG95 vaccine molecule. These results have marked implications for future explorations of aspects such as developmentally-regulated gene transcription/expression (using replicate samples) for all E. granulosus stages; structural and functional roles of non-coding genome regions; molecular 'cross-talk' between oncosphere and the immune system; and defining the precise function(s) of EG95. Applied aspects should include developing improved tools for the diagnosis and chemotherapy of cystic echinococcosis of humans.
(© 2022. The Author(s).)
Databáze: MEDLINE
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