Reliability of point-of-care circulating cathodic antigen assay for diagnosing schistosomiasis mansoni in urine samples from an endemic area of Brazil after one year of storage at -20 degrees Celsius.

Autor:	Favre TC; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Educação em Ambiente e Saúde, Rio de Janeiro, RJ, Brasil., Beck LCNH; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Educação em Ambiente e Saúde, Rio de Janeiro, RJ, Brasil., Bezerra FSM; Universidade Federal do Ceará, Departamento de Análises Clínicas e Toxicológicas, Fortaleza, CE, Brasil., Graeff-Teixeira C; Universidade Federal do Espírito Santo, Centro de Ciências da Saúde, Unidade de Doenças Infecciosas, Vitória, ES, Brasil.; Pontifícia Universidade Católica do Rio Grande do Sul, Laboratório de Parasitologia Biomédica, Porto Alegre, RS, Brasil., Coelho PMZ; Fundação Oswaldo Cruz, Instituto René Rachou, Belo Horizonte, MG, Brasil., Enk MJ; Instituto Evandro Chagas, Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia, Secção de Parasitologia, Ananindeua, PA, Brasil., Katz N; Fundação Oswaldo Cruz, Instituto René Rachou, Belo Horizonte, MG, Brasil., Oliveira RR; Fundação Oswaldo Cruz, Instituto Gonçalo Moniz, Salvador, BA, Brasil., Reis MGD; Fundação Oswaldo Cruz, Instituto Gonçalo Moniz, Salvador, BA, Brasil.; Universidade Federal da Bahia, Faculdade de Medicina, Salvador, BA, Brasil.; Yale University, School of Public Health, Department of Epidemiology of Microbial Diseases, New Haven, CT, United States of America., Pieri OS; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Educação em Ambiente e Saúde, Rio de Janeiro, RJ, Brasil.
Jazyk:	angličtina
Zdroj:	Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2022 Feb 25; Vol. 55, pp. e0389. Date of Electronic Publication: 2022 Feb 25 (Print Publication: 2022).
DOI:	10.1590/0037-8682-0389-2021
Abstrakt:	Background: The World Health Organization recommends reliable point-of-care (POC) diagnostic testing to eliminate schistosomiasis. Lateral flow immunoassay that detects schistosome circulating cathodic antigen (CCA) in urine to establish prevalence thresholds for intervention in endemic areas is recommended. Stored urine may be useful if surveying at-risk populations is delayed or interrupted by unforeseen circumstances, such as the current COVID-19 pandemic. This study evaluated the manufacturer's claim that Schistosoma mansoni infection can be reliably diagnosed in urine samples stored at -20°C for one year. Methods: Two-hundred-forty-two subjects from an endemic site in Brazil provided one urine sample each for testing with URINE CCA (SCHISTO) ECO TESTE® (POC-ECO) and one stool sample each for testing with Kato-Katz (KK) and Helmintex® (HTX) as a robust reference standard for infection status. At least 2 ml of urine from each participant was stored at -20°C; after one year, 76 samples were randomly selected for POC-ECO retesting. Results: The POC-ECO agreement between freshly collected and stored urine was inadequate considering trace results as positive (Cohen's kappa coefficient κ = 0.08) and negative (κ = 0.36). POC-ECO accuracy was not significantly greater than that of routine KK (54%; 95% confidence interval: 42.1%-65.5%). Conclusions: The precision and accuracy of POC-ECO have to be optimized in both freshly collected and stored urine before it can be recommended for use in control programs in Brazil.
Databáze:	MEDLINE
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