Optical Clearing Agents for Optical Imaging Through Cartilage Tympanoplasties: A Preclinical Feasibility Study.

Autor:	Wang J; School of Biomedical Engineering, Dalhousie University., Chawdhary G; Department of Otolaryngology, Royal Hallamshire Hospital, Sheffield, U.K., Yang X; School of Biomedical Engineering, Dalhousie University.; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, China., Morin F; Family Medicine, McGill University, Montreal, Quebec., Khalid-Raja M; Stepping Hill Hospital, Stockport NHS Foundation Trust, Great Manchester, England, U.K., Farrell J; School of Biomedical Engineering, Dalhousie University., MacDougall D; School of Biomedical Engineering, Dalhousie University., Chen F; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, China., Morris DP; Division of Otolaryngology Head & Neck Surgery, Department of Surgery., Adamson RBA; School of Biomedical Engineering, Dalhousie University.; Division of Otolaryngology Head & Neck Surgery, Department of Surgery.; Electrical and Computer Engineering Department, Dalhousie University, Halifax, Nova Scotia, Canada.
Jazyk:	angličtina
Zdroj:	Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology [Otol Neurotol] 2022 Apr 01; Vol. 43 (4), pp. e467-e474.
DOI:	10.1097/MAO.0000000000003502
Abstrakt:	Hypothesis: Optical clearing agents (OCAs) can render cartilage tympanoplasty grafts sufficiently transparent to permit visualization of middle ear structures in an operated ear using optical coherence tomography (OCT) imaging. Methods: Pieces of human tragal cartilage were treated with glycerol, a commonly used OCA. A reference reflector was imaged with OCT through the tympanoplasty as it cleared and the optical attenuation of the graft was measured. The reversibility of clearing and the dimensional changes associated with glycerol absorption were also measured. In a separate experiment, a human cadaveric temporal bone was prepared to simulate an ossiculoplasty surgery with cartilage replacement of the tympanic membrane. A partial ossicular replacement prosthesis (PORP) inserted in the ear was imaged with OCT through a 0.4mm cartilage graft optically cleared with glycerol. Main Outcome Measure: The optical attenuation of 0.4mm cartilage grafts decreased at 2.3+/-1.1 dB/min following treatment with glycerol, reaching a total decrease in attenuation of 13.6+/-5.9 dB after 7 minutes. The optical and dimensional effects of glycerol absorption were reversable following saline washout. In the temporal bone preparation, treatment of a cartilage graft with glycerol resulted in a 13 dB increase in signal-to-noise ratio and a 13 dB increase in contrast for visualizing the PORP through the graft with OCT. Conclusions: Optical clearing agents offer a potential pathway towards optical coherence tomography imaging of the middle ear in post-surgical ears with cartilage grafts. Competing Interests: Conflicts of Interest and Source of Funding: J.W., J.F., D.M., and R.A. own equity in Audioptics Medical Inc., a start-up company working to commercialize middle ear optical coherence tomography technology. G.C., X.Y., F.M., M.K.-R., F.C., D.P.M. declare no conflict of interest. (Copyright © 2022, Otology & Neurotology, Inc.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu