Investigation of Platelet Aggregation in Atherosclerosis.

Autor:	Lordan R; Department of Biological Sciences, University of Limerick, Limerick, Ireland.; Health Research Institute, University of Limerick, Limerick, Ireland., Tsoupras A; Department of Biological Sciences, University of Limerick, Limerick, Ireland.; Health Research Institute, University of Limerick, Limerick, Ireland.; Bernal Institute, University of Limerick, Limerick, Ireland., Zabetakis I; Department of Biological Sciences, University of Limerick, Limerick, Ireland. Ioannis.Zabetakis@ul.ie.; Health Research Institute, University of Limerick, Limerick, Ireland. Ioannis.Zabetakis@ul.ie.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2419, pp. 333-347.
DOI:	10.1007/978-1-0716-1924-7_21
Abstrakt:	Platelet activation and aggregation is implicated in all stages of inflammation-related atherosclerosis from the initial steps of endothelial dysfunction and plaque formation, to plaque rupture and atherothrombotic events, such as acute coronary syndrome, myocardial infarction, and ischemic incidences. Platelet aggregometry assays are the mainstream for evaluating and monitoring platelet reactivity in such conditions and for the investigation of prophylactic and therapeutic approaches. The most established methodology is light transmittance aggregometry (LTA). Here we describe the appropriate preparation of platelet suspensions from human blood and the methodology of LTA-based assays that is used for basic and clinical research for monitoring and evaluating the activities of several thrombotic mediators, as well as determining the dose efficacy and safety of several pharmaceutical and nutraceutical compounds intended for therapeutic and prophylactic interventions for atherosclerosis. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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