Genome analysis of Legionella pneumophila ST23 from various countries reveals highly similar strains.

Autor:	Ricci ML; Department of Infectious Diseases Istituto Superiore di Sanità, Rome, Italy.; ESCMID Study Group for Legionella Infections (ESGLI), Basel, Switzerland., Fillo S; Scientific Department, Army Medical Center, Rome, Italy., Ciammaruconi A; Scientific Department, Army Medical Center, Rome, Italy., Lista F; Scientific Department, Army Medical Center, Rome, Italy., Ginevra C; CIRI, Centre International de Recherche en Infectiologie, Legionella Pathogenesis Team, University of Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Lyon, France; National Reference Centre of Legionella, Institute of Infectious Agents, Hospices Civils de Lyon, Lyon, France.; ESCMID Study Group for Legionella Infections (ESGLI), Basel, Switzerland., Jarraud S; Universitè Lyon 1, CNR Legionella, Lyon, France.; ESCMID Study Group for Legionella Infections (ESGLI), Basel, Switzerland., Girolamo A; Department of Infectious Diseases Istituto Superiore di Sanità, Rome, Italy., Barbanti F; Department of Infectious Diseases Istituto Superiore di Sanità, Rome, Italy., Rota MC; Department of Infectious Diseases Istituto Superiore di Sanità, Rome, Italy., Lindsay D; Scottish Microbiology Reference Laboratories, Glasgow, Scotland.; ESCMID Study Group for Legionella Infections (ESGLI), Basel, Switzerland., Gorzynski J; Scottish Microbiology Reference Laboratories, Glasgow, Scotland., Uldum SA; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen S, Denmark.; ESCMID Study Group for Legionella Infections (ESGLI), Basel, Switzerland., Baig S; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen S, Denmark., Foti M; Agency for Health Protection of Metropolitan Area of Milan (ATS), Milan, Italy., Petralito G; Scientific Department, Army Medical Center, Rome, Italy., Torri S; Department of Laboratory of Medicine, Hospital Niguarda, Ca' Granda, Milan, Italy., Faccini M; Agency for Health Protection of Metropolitan Area of Milan (ATS), Milan, Italy., Bonini M; Agency for Health Protection of Metropolitan Area of Milan (ATS), Milan, Italy., Gentili G; Agency for Health Protection of Metropolitan Area of Milan (ATS), Milan, Italy., Senatore S; Agency for Health Protection of Metropolitan Area of Milan (ATS), Milan, Italy., Lamberti A; Agency for Health Protection of Metropolitan Area of Milan (ATS), Milan, Italy., Carrico JA; Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.; ESCMID Study Group for Legionella Infections (ESGLI), Basel, Switzerland., Scaturro M; Department of Infectious Diseases Istituto Superiore di Sanità, Rome, Italy maria.scaturro@iss.it.; ESCMID Study Group for Legionella Infections (ESGLI), Basel, Switzerland.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2022 Mar 02; Vol. 5 (6). Date of Electronic Publication: 2022 Mar 02 (Print Publication: 2022).
DOI:	10.26508/lsa.202101117
Abstrakt:	Legionella pneumophila serogroup 1 (Lp1) sequence type (ST) 23 is one of the most commonly detected STs in Italy where it currently causes all investigated outbreaks. ST23 has caused both epidemic and sporadic cases between 1995 and 2018 and was analysed at genomic level and compared with ST23 isolated in other countries to determine possible similarities and differences. A core genome multi-locus sequence typing (cgMLST), based on a previously described set of 1,521 core genes, and single-nucleotide polymorphisms (SNPs) approaches were applied to an ST23 collection including genomes from Italy, France, Denmark and Scotland. DNAs were automatically extracted, libraries prepared using NextEra library kit and MiSeq sequencing performed. Overall, 63 among clinical and environmental Italian Lp1 isolates and a further seven and 11 ST23 from Denmark and Scotland, respectively, were sequenced, and pangenome analysed. Both cgMLST and SNPs analyses showed very few loci and SNP variations in ST23 genomes. All the ST23 causing outbreaks and sporadic cases in Italy and elsewhere, were phylogenetically related independent of year, town or country of isolation. Distances among the ST23s were further shortened when SNPs due to horizontal gene transfers were removed. The Lp1 ST23 isolated in Italy have kept their monophyletic origin, but they are phylogenetically close also to ST23 from other countries. The ST23 are quite widespread in Italy, and a thorough epidemiological investigation is compelled to determine sources of infection when this ST is identified in both LD sporadic cases and outbreaks. (© 2022 Ricci et al.)
Databáze:	MEDLINE
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