Cobalamin cbiP mutant shows decreased tolerance to low temperature and copper stress in Listeria monocytogenes.
Autor: | Vásquez L; Laboratorio de Microbiología y Probióticos, INTA Universidad de Chile, Avenida El Líbano 5524 Macul, Santiago, Chile., Parra A; Doctorado en Acuicultura, Programa Cooperativo Universidad de Chile, Universidad Católica del Norte, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.; Facultad de Ciencias del Mar, Universidad Católica del Norte, Larrondo 1281, Coquimbo, Chile., Quesille-Villalobos AM; Laboratorio de Microbiología y Probióticos, INTA Universidad de Chile, Avenida El Líbano 5524 Macul, Santiago, Chile., Gálvez G; Laboratorio de Bioingeniería, Instituto de Ciencias de la Ingeniería, Universidad de O'Higgins, Rancagua, Chile., Navarrete P; Laboratorio de Microbiología y Probióticos, INTA Universidad de Chile, Avenida El Líbano 5524 Macul, Santiago, Chile.; ANID-Millennium Science Initiative Program-Millennium Nucleus in the Biology of the Intestinal Microbiota, Santiago, Chile., Latorre M; Laboratorio de Bioingeniería, Instituto de Ciencias de la Ingeniería, Universidad de O'Higgins, Rancagua, Chile.; Laboratorio de Bioinformática y Expresión Génica, INTA, Universidad de Chile, Santiago, Chile., Toro M; Laboratorio de Microbiología y Probióticos, INTA Universidad de Chile, Avenida El Líbano 5524 Macul, Santiago, Chile., González M; Laboratorio de Bioinformática y Expresión Génica, INTA, Universidad de Chile, Santiago, Chile.; Fondap Center for Genome Regulation (CGR), Santiago, Chile., Reyes-Jara A; Laboratorio de Microbiología y Probióticos, INTA Universidad de Chile, Avenida El Líbano 5524 Macul, Santiago, Chile. areyes@inta.uchile.cl. |
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Jazyk: | angličtina |
Zdroj: | Biological research [Biol Res] 2022 Mar 02; Vol. 55 (1), pp. 9. Date of Electronic Publication: 2022 Mar 02. |
DOI: | 10.1186/s40659-022-00376-4 |
Abstrakt: | Background: Listeria monocytogenes is a foodborne pathogen that causes listeriosis in humans. This pathogen activates multiple regulatory mechanisms in response to stress, and cobalamin biosynthesis might have a potential role in bacterial protection. Low temperature is a strategy used in the food industry to control bacteria proliferation; however, L. monocytogenes can grow in cold temperatures and overcome different stress conditions. In this study we selected L. monocytogenes List2-2, a strain with high tolerance to the combination of low temperature + copper, to understand whether the cobalamin biosynthesis pathway is part of the tolerance mechanism to this stress condition. For this, we characterized the transcription level of three cobalamin biosynthesis-related genes (cbiP, cbiB, and cysG) and the eutV gene, a transcriptional regulator encoding gene involved in ethanolamine metabolism, in L. monocytogenes strain List2-2 growing simultaneously under two environmental stressors: low temperature (8 °C) + copper (0.5 mM of CuSO Results: By analyzing the KEGG pathway database, twenty-two genes were involved in the cobalamin biosynthesis pathway in L. monocytogenes List2-2. The expression of genes cbiP, cbiB, and cysG, and eutV increased 6 h after the exposure to low temperature + copper. The cobalamin cbiP mutant strain List2-2ΔcbiP showed less tolerance to low temperature + copper (3 mM) than the wild-type L. monocytogenes List2-2. The addition of cyanocobalamin (5 nM) to the medium reverted the phenotype observed in List2-2ΔcbiP. Conclusion: These results indicate that cobalamin biosynthesis is necessary for L. monocytogenes growth under stress and that the cbiP gene may play a role in the survival and growth of L. monocytogenes List2-2 at low temperature + copper. (© 2022. The Author(s).) |
Databáze: | MEDLINE |
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