Identification of a discrete subpopulation of spinal cord ependymal cells with neural stem cell properties.

Autor:	Stenudd M; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Sabelström H; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Llorens-Bobadilla E; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Zamboni M; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Blom H; Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, 171 21 Solna, Sweden., Brismar H; Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, 171 21 Solna, Sweden., Zhang S; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Basak O; Hubrecht Institute for Developmental Biology and Stem Cell Research, 3584 CT Utrecht, the Netherlands; University Medical Centre Utrecht, 3584 GC, Utrecht, the Netherlands., Clevers H; Hubrecht Institute for Developmental Biology and Stem Cell Research, 3584 CT Utrecht, the Netherlands; University Medical Centre Utrecht, 3584 GC, Utrecht, the Netherlands., Göritz C; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden; Ming Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institutet, 171 77 Stockholm, Sweden., Barnabé-Heider F; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden; Department of Neuroscience, Karolinska Institutet, 171 77 Stockholm, Sweden., Frisén J; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden. Electronic address: jonas.frisen@ki.se.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2022 Mar 01; Vol. 38 (9), pp. 110440.
DOI:	10.1016/j.celrep.2022.110440
Abstrakt:	Spinal cord ependymal cells display neural stem cell properties in vitro and generate scar-forming astrocytes and remyelinating oligodendrocytes after injury. We report that ependymal cells are functionally heterogeneous and identify a small subpopulation (8% of ependymal cells and 0.1% of all cells in a spinal cord segment), which we denote ependymal A (EpA) cells, that accounts for the in vitro stem cell potential in the adult spinal cord. After spinal cord injury, EpA cells undergo self-renewing cell division as they give rise to differentiated progeny. Single-cell transcriptome analysis revealed a loss of ependymal cell gene expression programs as EpA cells gained signaling entropy and dedifferentiated to a stem-cell-like transcriptional state after an injury. We conclude that EpA cells are highly differentiated cells that can revert to a stem cell state and constitute a therapeutic target for spinal cord repair. Competing Interests: Declaration of interests J.F., E.L.-B., and M.Z. are consultants to 10x Genomics. (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu