Active indole-3-acetic acid biosynthesis by the bacterium Azospirillum brasilense cultured under a biogas atmosphere enables its beneficial association with microalgae.

Autor: Barbosa-Nuñez JA; Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Guadalajara, Mexico., Palacios OA; Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Chihuahua, Mexico.; Centro de Investigaciones Biológicas del Noroeste (CIBNOR), La Paz, Mexico., de-Bashan LE; Centro de Investigaciones Biológicas del Noroeste (CIBNOR), La Paz, Mexico.; The Bashan Institute of Science, Auburn, Alabama, USA.; Departament of Entomology and Plant Pathology, Auburn University, Auburn, Alabama, USA., Snell-Castro R; Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Guadalajara, Mexico., Corona-González RI; Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Guadalajara, Mexico., Choix FJ; Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Chihuahua, Mexico.; CONACYT - Universidad Autónoma de Chihuahua, Chihuahua, Mexico.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2022 May; Vol. 132 (5), pp. 3650-3663. Date of Electronic Publication: 2022 Mar 15.
DOI: 10.1111/jam.15509
Abstrakt: Aims: This study assessed, at the physiological and molecular levels, the effect of biogas on indole-3-acetic acid (IAA) biosynthesis by Azospirillum brasilense as well as the impact of this bacterium during CO 2 fixation from biogas by Chlorella vulgaris and Scenedesmus obliquus.
Methods and Results: IpdC gene expression, IAA production and the growth of A. brasilense cultured under air (control) and biogas (treatment) were evaluated. The results demonstrated that A. brasilense had a better growth capacity and IAA production (105.7 ± 10.3 μg ml -1 ) when cultured under biogas composed of 25% CO 2  + 75% methane (CH 4 ) with respect to the control (72.4 ± 7.9 μg ml -1 ), although the ipdC gene expression level was low under the stressful condition generated by biogas. Moreover, this bacterium was able to induce a higher cell density and CO 2 fixation rate from biogas by C. vulgaris (0.27 ± 0.08 g l -1 d -1 ) and S. obliquus (0.22 ± 0.08 g l -1 d -1 ).
Conclusions: This study demonstrated that A. brasilense has the capacity to grow and actively maintain its main microalgal growth-promoting mechanism when cultured under biogas and positively influence CO 2 fixation from the biogas of C. vulgaris and S. obliquus.
Significance and Impact of the Study: These findings broaden research in the field of Azospirillum-microalga interactions and the prevalence of Azospirillum in environmental and ecological topics in addition to supporting the uses of plant growth-promoting bacteria to enhance biotechnological strategies for biogas upgrading.
(© 2022 The Society for Applied Microbiology.)
Databáze: MEDLINE
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