Oxidative imbalance and muscular alterations in diverticular disease.

Autor: Pallotta L; Department of Translational and Precision Medicine, Sapienza University of Rome, Italy. Electronic address: lucia.pallotta@uniroma1.it., Vona R; Center for Gender-Specific Medicine, Italian National Institute of Health, Rome, Italy., Maselli MA; Experimental Pharmacology Laboratory, National Institute of Gastroenterology 'S. de Bellis', Research Hospital, Castellana Grotte, BA, USA., Cicenia A; Department of Translational and Precision Medicine, Sapienza University of Rome, Italy., Bella A; Department of Infectious Diseases, Italian National Institute of Health, Rome, Italy., Ignazzi A; Experimental Pharmacology Laboratory, National Institute of Gastroenterology 'S. de Bellis', Research Hospital, Castellana Grotte, BA, USA., Carabotti M; Medical-Surgical Department of Clinical Sciences and Translational Medicine, Sapienza University of Rome, Italy., Cappelletti M; Department of Translational and Precision Medicine, Sapienza University of Rome, Italy., Gioia A; Department of Translational and Precision Medicine, Sapienza University of Rome, Italy., Tarallo M; Department of Surgery 'P. Valdoni', Sapienza University of Rome, Italy., Tellan G; Department of Internistic, Anaesthetic and Cardiovascular Clinical Sciences, Sapienza University of Rome, Italy., Fiori E; Department of Surgery 'P. Valdoni', Sapienza University of Rome, Italy., Pezzolla F; Experimental Pharmacology Laboratory, National Institute of Gastroenterology 'S. de Bellis', Research Hospital, Castellana Grotte, BA, USA., Matarrese P; Center for Gender-Specific Medicine, Italian National Institute of Health, Rome, Italy., Severi C; Department of Translational and Precision Medicine, Sapienza University of Rome, Italy.
Jazyk: angličtina
Zdroj: Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver [Dig Liver Dis] 2022 Sep; Vol. 54 (9), pp. 1186-1194. Date of Electronic Publication: 2022 Feb 26.
DOI: 10.1016/j.dld.2022.02.001
Abstrakt: Background: It is still a matter of debate if neuromuscular alterations reflect a primary event in diverticular disease (DD).
Aims: This study aimed to assess colonic wall layers from both stenotic and non-stenotic complicated DD, bio-phenotypic alterations, inflammatory and oxidative status.
Methods: A systematic analysis of colonic specimens obtained from stenotic and non-stenotic DD specimens was conducted and compared with controls. Biological activity and qPCR analysis were performed on longitudinal and circular muscles. Western blot analysis was performed throughout colonic wall layers to quantify oxidative and inflammatory markers.
Results: A homogenous increase in oxidative stress was observed through all the layers, which were more sharpened in the longitudinal muscle for a loss in antioxidant defenses. In both stenotic and non-stenotic colon, the longitudinal muscle presented an impaired relaxation and a cellular phenotypic switch driven by transforming growth factor-β with an increase in mRNA expression of collagen Iα and a decrease in myosin heavy chain. The circular muscle, as the mucosa, was less affected by molecular alterations. No peculiar increase in inflammatory markers was observed.
Conclusion: A longitudinal colonic myopathy is present in DD, independently from the disease stage associated with an oxidative imbalance that could suggest new therapeutic strategies.
Competing Interests: Declaration of Competing Interest None declared.
(Copyright © 2022 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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