Inexpensive portable capillary electrophoresis instrument for Monitoring Zinc(II) in remote areas.

Autor: Zhang M; School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences-Chemistry, University of Tasmania, Private Bag 75, Hobart, TAS 7001, Australia. Electronic address: zhangmin@guet.edu.cn., Smejkal P; Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences-Chemistry, University of Tasmania, Private Bag 75, Hobart, TAS 7001, Australia. Electronic address: petr.smejkal@utas.edu.au., Bester N; Sandy Bay, Hobart, TAS 7005, Australia., Robertson JC; Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences-Chemistry, University of Tasmania, Private Bag 75, Hobart, TAS 7001, Australia. Electronic address: johnathon.robertson@utas.edu.au., Atia MA; Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences-Chemistry, University of Tasmania, Private Bag 75, Hobart, TAS 7001, Australia. Electronic address: Mostafa.Adel@utas.edu.au., Townsend AT; Central Science Laboratory, University of Tasmania, Private Bag 74, Hobart, TAS 7001, Australia. Electronic address: Ashley.Townsend@utas.edu.au., Guijt RM; Centre for Regional and Rural Futures, Deakin University, 75 Pigdons Road, Waurn Ponds, VIC 3216, Australia. Electronic address: Rosanne.Guijt@deakin.edu.au., Breadmore MC; Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences-Chemistry, University of Tasmania, Private Bag 75, Hobart, TAS 7001, Australia. Electronic address: Michael.Breadmore@utas.edu.au.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2022 Apr 12; Vol. 1668, pp. 462895. Date of Electronic Publication: 2022 Feb 11.
DOI: 10.1016/j.chroma.2022.462895
Abstrakt: A compact, inexpensive capillary electrophoresis instrument was developed for monitoring metal ions and evaluated for Zn(II) in remote contaminated locations in western Tasmania, Australia. The portable instrument, measuring 21 cm x 10 cm x 7 cm, was powered from the USB port of a laptop computer and built from off-the-shelf components costing ∼$1200 USD. Electrophoretic separations were conducted using a fused silica capillary (10-50 µm I.D.), applying 8.5 kV over capillaries ranging from 25 cm to 40 cm in length. The capillary inlet was connected with an electrically grounded cross-piece as flow-through injection interface. Automated fluidic management was achieved by controlling four mini peristaltic pumps and a solenoid valve. Detection was realised using a purpose-built visible LED absorption detector, optimised for the detection of Co(II), Cu(II) and Zn(II) after complexation with 4-(2-Pyridylazo) resorcinol (PAR). Limits of detection of sub-µM were obtained. The instrument was tested for continuous operation in the laboratory for up to 3 months, and relative standard deviations of <5.4% were found over 945 consecutive injections. In the field, the system was able to measure 106 samples within 11 h, the time it can be powered from the laptop computer. As Field measurement of Zn(II) in western Tasmania was demonstrated to show capability for on-site metal testing.
Competing Interests: Declaration of Competing Interest The authors declare no competing financial interest.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
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