The ER membrane complex (EMC) can functionally replace the Oxa1 insertase in mitochondria.

Autor: Güngör B; Cell Biology, University of Kaiserslautern, Kaiserslautern, Germany., Flohr T; Cell Biology, University of Kaiserslautern, Kaiserslautern, Germany., Garg SG; Institute for Molecular Evolution, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany., Herrmann JM; Cell Biology, University of Kaiserslautern, Kaiserslautern, Germany.
Jazyk: angličtina
Zdroj: PLoS biology [PLoS Biol] 2022 Mar 01; Vol. 20 (3), pp. e3001380. Date of Electronic Publication: 2022 Mar 01 (Print Publication: 2022).
DOI: 10.1371/journal.pbio.3001380
Abstrakt: Two multisubunit protein complexes for membrane protein insertion were recently identified in the endoplasmic reticulum (ER): the guided entry of tail anchor proteins (GET) complex and ER membrane complex (EMC). The structures of both of their hydrophobic core subunits, which are required for the insertion reaction, revealed an overall similarity to the YidC/Oxa1/Alb3 family members found in bacteria, mitochondria, and chloroplasts. This suggests that these membrane insertion machineries all share a common ancestry. To test whether these ER proteins can functionally replace Oxa1 in yeast mitochondria, we generated strains that express mitochondria-targeted Get2-Get1 and Emc6-Emc3 fusion proteins in Oxa1 deletion mutants. Interestingly, the Emc6-Emc3 fusion was able to complement an Δoxa1 mutant and restored its respiratory competence. The Emc6-Emc3 fusion promoted the insertion of the mitochondrially encoded protein Cox2, as well as of nuclear encoded inner membrane proteins, although was not able to facilitate the assembly of the Atp9 ring. Our observations indicate that protein insertion into the ER is functionally conserved to the insertion mechanism in bacteria and mitochondria and adheres to similar topological principles.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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