Rescue of photoreceptor with human mesenchyme stem cell and human mesenchyme stem cell expressing erythropoietin in total degeneration of retina animal model.

Autor: Abd Rashid M; Department of Ophthalmology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM) Medical Centre, Sakaka, Al-Jawf Province, Saudi Arabia., Chenshen L; Department of Ophthalmology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM) Medical Centre, Sakaka, Al-Jawf Province, Saudi Arabia., Ee Hwan AK; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Al-Jawf Province, Saudi Arabia; Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia., AlSaeedy H; Department of Medical Microbiology, Universiti Putra Malaysia, Serdang, Malaysia., Mok PL; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Al-Jawf Province, Saudi Arabia; Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia., Subbiah SK; Department of Medical Microbiology, Universiti Putra Malaysia, Serdang, Malaysia; Centre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Chennai, India., Isa HM; Department of Ophthalmology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM) Medical Centre, Sakaka, Al-Jawf Province, Saudi Arabia., Yong TK; Department of Ophthalmology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM) Medical Centre; Brighton Healthcare, UKM Medical Centre, Cheras, Kuala Lumpur, Malaysia., Nizam Harun MH; Department of Ophthalmology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM) Medical Centre, Sakaka, Al-Jawf Province, Saudi Arabia., B S Mohamad SMS; Department of Ophthalmology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM) Medical Centre, Sakaka, Al-Jawf Province, Saudi Arabia., Min Hwei AN; Tissue Engineering Centre, UKM Medical Centre, Cheras, Kuala Lumpur,, Malaysia., Luu CD; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital; Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, Australia., Catherine Bastion ML; Department of Ophthalmology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM) Medical Centre, Sakaka, Al-Jawf Province, Saudi Arabia.
Jazyk: angličtina
Zdroj: Indian journal of ophthalmology [Indian J Ophthalmol] 2022 Mar; Vol. 70 (3), pp. 921-929.
DOI: 10.4103/ijo.IJO_472_21
Abstrakt: Purpose: This study aimed to investigate the efficacy of human-derived umbilical cord mesenchymal stem cells (HDUMSC) and human-derived umbilical cord mesenchymal stem cells expressing erythropoietin (HDUMSC-EPO) to rescue total degenerated retina in a rat model.
Methods: The study included four treatment groups, namely negative control using normal saline (HBSS) injection, positive control using sodium iodide 60 mg/kg (SI), SI treated with HDUMSC, and SI treated with HDUMSC-EPO given via subretinal and intravenous routes, to test the efficacy of retinal regeneration following SI-induced retinal degeneration. Retinal function in both phases was tested via electroretinography (ERG) and histological staining examining the outer nuclear layer (ONL).
Results: There was a statistically significant result (P < 0.05) in the SI treated with HDUMSC-EPO only when comparing day 11 (mean = 23.6 μv), day 18 (mean = 25.2 μv), day 26 (mean = 26.3 μv), and day 32 (mean = 28.2 μv) to the b-wave ERG on day 4 rescue injection day (mean = 12.5 μv). The SI treated with HDUMSC-EPO showed significant improvement in b-wave ERG readings in the Sprague-Dawley (SD) rat but did not restore baseline readings prior to degeneration (day 0). Both treated groups' ONL thicknesses did not show significant changes compared to the negative control group (HBSS) following rescue therapy.
Conclusion: Total retinal degeneration following intravenous SI injection was observed at 60 mg/kg. SI treated with HDUMSC and HDUMSC-EPO showed no regenerative potential compared to baseline in SI-induced total retina degeneration on ERG or histology, whereas SI treated with HDUMSC-EPO group showed a substantial increase in b-wave ERG amplitude over time.
Competing Interests: None
Databáze: MEDLINE
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