Application of Congo red dye as a molecular probe to investigate the kinetics and thermodynamics of the formation processes of arachin and conarachin nanocomplexes.

Autor: Rezende JP; Applied Molecular Thermodynamics Group (THERMA), Department of Food Technology, Federal University of Viçosa, Av. P. H. Rolfs s/n, 36570900 Viçosa, MG, Brazil., De Paula HMC; Colloidal and Macromolecular Green Chemistry Group (QUIVECOM), Department of Chemistry, Federal University of Viçosa, Av. P. H. Rolfs s/n, 36570900 Viçosa, MG, Brazil., Freitas TD; Applied Molecular Thermodynamics Group (THERMA), Department of Food Technology, Federal University of Viçosa, Av. P. H. Rolfs s/n, 36570900 Viçosa, MG, Brazil., Coelho YL; Colloidal and Macromolecular Green Chemistry Group (QUIVECOM), Department of Chemistry, Federal University of Viçosa, Av. P. H. Rolfs s/n, 36570900 Viçosa, MG, Brazil., Da Silva LHM; Colloidal and Macromolecular Green Chemistry Group (QUIVECOM), Department of Chemistry, Federal University of Viçosa, Av. P. H. Rolfs s/n, 36570900 Viçosa, MG, Brazil. Electronic address: luhen@ufv.br., Pires ACDS; Applied Molecular Thermodynamics Group (THERMA), Department of Food Technology, Federal University of Viçosa, Av. P. H. Rolfs s/n, 36570900 Viçosa, MG, Brazil. Electronic address: ana.pires@ufv.br.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2022 Aug 01; Vol. 384, pp. 132485. Date of Electronic Publication: 2022 Feb 16.
DOI: 10.1016/j.foodchem.2022.132485
Abstrakt: The thermodynamics and kinetics of arachin-Congo red (ARA-CR) and conarachin-Congo red (CON-CR) interactions were studied using surface plasmon resonance. KCl led to a reduction of up to 55% in the values of the associated kinetic constants, but it had less influence on the dissociation rates (less than 12%). The change in ionic strength had little effect on the thermodynamic stability of the complexes, but it did reduce their affinities ( [Formula: see text] from 3.52 to 2.44 × 10 3  M -1 and [Formula: see text] from 15.1 to 12.5 × 10 3  M -1 ). The shielding of the electrical double layer favored ARA-CR hydrophilic interactions ( [Formula: see text] decreased from -30.60 to -42.98 kJ mol -1 ). On the other hand, hydrophobic interactions came to dominate during the formation of [CON-CR] 0 ( [Formula: see text] increased from -11.21 to 28.34 kJ mol -1 and [Formula: see text] increased from 12.64 to 51.73 kJ.mol -1 ). The data presented here improve our understanding of plant-based protein nanocarriers of small bioactive molecules.
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Databáze: MEDLINE
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