Azadirachta indica A. Juss bark extract and its Nimbin isomers restrict β-coronaviral infection and replication.

Autor: Sarkar L; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, India., Oko L; Department of Neurology, University of Colorado School of Medicine, Aurora, CO, 80045, USA., Gupta S; The Systems Virology Lab, Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden., Bubak AN; Department of Neurology, University of Colorado School of Medicine, Aurora, CO, 80045, USA., Das B; Department of Chemical Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, India., Gupta P; Department of Chemical Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, India., Safiriyu AA; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, India., Singhal C; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, India., Neogi U; The Systems Virology Lab, Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden., Bloom D; Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL, USA., Banerjee A; Laboratory of Virology, Regional Centre for Biotechnology, and Translational Health Science & Technology Institute Faridabad, Haryana, India., Mahalingam R; Department of Neurology, University of Colorado School of Medicine, Aurora, CO, 80045, USA., Cohrs RJ; Department of Neurology, University of Colorado School of Medicine, Aurora, CO, 80045, USA., Koval M; Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, Emory University School of Medicine, Atlanta, GA, 30322, USA., Shindler KS; Departments of Ophthalmology and Neurology, University of Pennsylvania, Scheie Eye Institute, Philadelphia, PA, USA., Pal D; Department of Computational and Data Sciences, Indian Institute of Science, Bangalore Karnataka, 560012, India., Nagel M; Department of Neurology, University of Colorado School of Medicine, Aurora, CO, 80045, USA., Sarma JD; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, India; Department of Ophthalmology, University of Pennsylvania, Philadelphia, PA, USA. Electronic address: dassarmaj@iiserkol.ac.in.
Jazyk: angličtina
Zdroj: Virology [Virology] 2022 Apr; Vol. 569, pp. 13-28. Date of Electronic Publication: 2022 Feb 15.
DOI: 10.1016/j.virol.2022.01.002
Abstrakt: Emerging mutations in the SARS-CoV-2 genome pose a challenge for vaccine development and antiviral therapy. The antiviral efficacy of Azadirachta indica bark extract (NBE) was assessed against SARS-CoV-2 and m-CoV-RSA59 infection. Effects of in vivo intranasal or oral NBE administration on viral load, inflammatory response, and histopathological changes were assessed in m-CoV-RSA59-infection. NBE administered inhibits SARS-CoV-2 and m-CoV-RSA59 infection and replication in vitro, reducing Envelope and Nucleocapsid gene expression. NBE ameliorates neuroinflammation and hepatitis in vivo by restricting viral replication and spread. Isolated fractions of NBE enriched in Nimbin isomers shows potent inhibition of m-CoV-RSA59 infection in vitro. In silico studies revealed that NBE could target Spike and RdRp of m-CoV and SARS-CoV-2 with high affinity. NBE has a triterpenoids origin that may allow them to competitively target panoply of viral proteins to inhibit mouse and different strains of human coronavirus infections, suggesting its potential as an antiviral against pan-β-Coronaviruses.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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