Lower polyunsaturated fatty acid levels and FADS2 expression in adult compared to neonatal keratinocytes are associated with FADS2 promotor hypermethylation.

Autor: Pararasa C; College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK., Messenger DJ; Unilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, MK44 1LQ, UK., Barrett KE; Unilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, MK44 1LQ, UK., Hyliands D; Unilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, MK44 1LQ, UK., Talbot D; Unilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, MK44 1LQ, UK., Fowler MI; Unilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, MK44 1LQ, UK., Kawatra T; Unilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, MK44 1LQ, UK., Gunn DA; Unilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, MK44 1LQ, UK., Lim FL; Unilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, MK44 1LQ, UK., Wainwright LJ; Unilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, MK44 1LQ, UK., Jenkins G; Unilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, MK44 1LQ, UK., Griffiths HR; College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK; Faculty of Medicine, Health and Life Sciences, Swansea University, SA2 8PP, UK. Electronic address: h.r.griffiths@swansea.ac.uk.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2022 Apr 23; Vol. 601, pp. 9-15. Date of Electronic Publication: 2022 Feb 22.
DOI: 10.1016/j.bbrc.2022.02.055
Abstrakt: Keratinocytes produce lipids that are critical for the skin barrier, however, little is known about the impact of age on fatty acid (FA) biosynthesis in these cells. We have examined the relationship between keratinocyte FA composition, lipid biosynthetic gene expression, gene promoter methylation and age. Expression of elongase (ELOVL6 and 7) and desaturase (FADS1 and 2) genes was lower in adult versus neonatal keratinocytes, and was associated with lower concentrations of n-7, n-9 and n-10 polyunsaturated FA in adult cells. Consistent with these findings, transient FADS2 knockdown in neonatal keratinocytes mimicked the adult keratinocyte FA profile in neonatal cells. Interrogation of methylation levels across the FADS2 locus (53 genomic sites) revealed differential methylation of 15 sites in neonatal versus adult keratinocytes, of which three hypermethylated sites in adult keratinocytes overlapped with a SMARCA4 protein binding site in the FADS2 promoter.
Competing Interests: Declaration of competing interest Messenger D.J.(2), Barrett K.E.(2), Hyliands D.(2), Talbot D.(2), Fowler M.I.(2), Kawatra T.(2), Gunn D.A.(2), Lim FL.(2), Wainwright L.J.(2), Jenkins G.(2), are employed by Unilever R&D. Griffiths H.R.(1,3) received part funding from Unilever R&D and BBSRC to undertake this study.
(Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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