Quantifiable Intravital Light Sheet Microscopy.

Autor:	Gibbs HC; Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA. hgibbs@tamu.edu.; Microscopy and Imaging Center, Texas A&M University, College Station, TX, USA. hgibbs@tamu.edu., Sarasamma S; Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA., Benavides OR; Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA., Green DG; Department of Cell and Systems Biology, University of Toronto, Toronto, Canada., Hart NA; Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA., Yeh AT; Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA., Maitland KC; Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA.; Microscopy and Imaging Center, Texas A&M University, College Station, TX, USA., Lekven AC; Department of Biology and Biochemistry, University of Houston, Houston, TX, USA.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2440, pp. 181-196.
DOI:	10.1007/978-1-0716-2051-9_11
Abstrakt:	Live imaging of zebrafish embryos that maintains normal development can be difficult to achieve due to a combination of sample mounting, immobilization, and phototoxicity issues that, once overcome, often still results in image quality sufficiently poor that computer-aided analysis or even manual analysis is not possible. Here, we describe our mounting strategy for imaging the zebrafish midbrain-hindbrain boundary (MHB) with light sheet fluorescence microscopy (LSFM) and pilot experiments to create a study-specific set of parameters for semiautomatically tracking cellular movements in the embryonic midbrain primordium during zebrafish segmentation. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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