Antibacterial activity of eugenol on the IS-58 strain of Staphylococcus aureus resistant to tetracycline and toxicity in Drosophila melanogaster.

Autor: Bezerra SR; Laboratory of Bioprospecting in the Semi-Arid and Alternative Methods (LABSEMA), Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil., Bezerra AH; Laboratory of Bioprospecting in the Semi-Arid and Alternative Methods (LABSEMA), Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil., de Sousa Silveira Z; Laboratory of Bioprospecting in the Semi-Arid and Alternative Methods (LABSEMA), Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil; Biological Sciences Pos Graduate Program - PPGCB, Federal University of Pernambuco - UFPE, Recife, 50670-901, PE, Brazil., Macedo NS; Laboratory of Bioprospecting in the Semi-Arid and Alternative Methods (LABSEMA), Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil; Biological Sciences Pos Graduate Program - PPGCB, Federal University of Pernambuco - UFPE, Recife, 50670-901, PE, Brazil., Dos Santos Barbosa CR; Laboratory of Bioprospecting in the Semi-Arid and Alternative Methods (LABSEMA), Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil; Biological Chemistry Pos Graduate Program, Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil., Muniz DF; Laboratory of Microbiology and Molecular Biology (LMBM), Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil., Sampaio Dos Santos JF; Laboratory of Bioprospecting in the Semi-Arid and Alternative Methods (LABSEMA), Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil; Biological Chemistry Pos Graduate Program, Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil., Melo Coutinho HD; Biological Chemistry Pos Graduate Program, Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil; Laboratory of Microbiology and Molecular Biology (LMBM), Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil. Electronic address: hdmcoutinho@urca.br., Bezerra da Cunha FA; Laboratory of Bioprospecting in the Semi-Arid and Alternative Methods (LABSEMA), Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil; Biological Chemistry Pos Graduate Program, Regional University of Cariri - URCA, Crato, 63105-000, CE, Brazil.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2022 Mar; Vol. 164, pp. 105456. Date of Electronic Publication: 2022 Feb 23.
DOI: 10.1016/j.micpath.2022.105456
Abstrakt: The indiscriminate use of antibiotics contributes significantly to the selection of bacteria resistant to several antibiotics. Among the resistance mechanisms are the Efflux Pumps which are responsible for extruding solutes from the cell cytoplasm through proteins in the cell membrane. Because of this, new strategies are needed to control multidrug-resistant pathogenic strains. In this way, the objective of this study was to evaluate the antibacterial activity of eugenol by inhibition of TetK Efflux Pump in strains of Staphylococcus aureus resistant to Tetracycline, in addition to evaluating its toxicity in Drosophila melanogaster. To determine the Minimum Inhibitory Concentration (MIC), the broth microdilution method was used. The modulated effect of antibiotic and Ethidium Bromide associated with eugenol in subinhibitory concentrations (MIC/8) was evaluated. To evaluate the toxic effect of eugenol on D. melanogaster, fumigation tests were used, in which the parameters of mortality and damage to the locomotor system were evaluated. The results showed that eugenol has no direct activity in S. aureus, with an MIC ≥1024 μg/mL. However, it demonstrated that the synergistic potential when associated with Tetracycline, reducing the MIC of the antibiotic, already associated with Ethidium Bromide, had an antagonistic effect. When the toxicity in D. melanogaster was evaluated, eugenol demonstrated a non-toxic profile, since it presented EC50: 2036 μL/mL in 48 h of exposure. In conclusion, eugenol had no relevant direct effect against S. aureus, however, it potentialized the action of the antibiotic by decreasing its MIC.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: