Rapid SARS-CoV-2 Intra-Host and Within-Household Emergence of Novel Haplotypes.

Autor: Manuto L; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy., Grazioli M; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy., Spitaleri A; Center for Omics Sciences, IRCCS San Raffaele Institute, 20132 Milan, Italy., Fontana P; Research and Innovation Center, Edmund Mach Foundation, 38098 San Michele all'Adige, Italy., Bianco L; Research and Innovation Center, Edmund Mach Foundation, 38098 San Michele all'Adige, Italy., Bertolotti L; Department of Veterinary Sciences, University of Torino, Grugliasco, 10095 Turin, Italy., Bado M; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy., Mazzotti G; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy., Bianca F; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy., Onelia F; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy., Lorenzin G; Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy., Simeoni F; Center for Omics Sciences, IRCCS San Raffaele Institute, 20132 Milan, Italy., Lazarevic D; Center for Omics Sciences, IRCCS San Raffaele Institute, 20132 Milan, Italy., Franchin E; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy.; Microbiology and Virology Diagnostic Unit, Padua University Hospital, Via Giustiniani, 35121 Padova, Italy., Del Vecchio C; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy.; Microbiology and Virology Diagnostic Unit, Padua University Hospital, Via Giustiniani, 35121 Padova, Italy., Dorigatti I; MRC Centre for Global Infectious Disease Analysis, Imperial College London, London SW7 2BX, UK., Tonon G; Center for Omics Sciences, IRCCS San Raffaele Institute, 20132 Milan, Italy.; Functional Genomics of Cancer Unit, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy., Cirillo DM; Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy., Lavezzo E; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy., Crisanti A; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy.; Microbiology and Virology Diagnostic Unit, Padua University Hospital, Via Giustiniani, 35121 Padova, Italy.; Department of Life Sciences, Imperial College London, London SW7 2BX, UK., Toppo S; Department of Molecular Medicine, University of Padova, 35121 Padua, Italy.; CRIBI Biotech Center, University of Padova, 35121 Padova, Italy.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2022 Feb 15; Vol. 14 (2). Date of Electronic Publication: 2022 Feb 15.
DOI: 10.3390/v14020399
Abstrakt: In February 2020, the municipality of Vo', a small town near Padua (Italy) was quarantined due to the first coronavirus disease 19 (COVID-19)-related death detected in Italy. To investigate the viral prevalence and clinical features, the entire population was swab tested in two sequential surveys. Here we report the analysis of 87 viral genomes, which revealed that the unique ancestor haplotype introduced in Vo' belongs to lineage B, carrying the mutations G11083T and G26144T. The viral sequences allowed us to investigate the viral evolution while being transmitted within and across households and the effectiveness of the non-pharmaceutical interventions implemented in Vo'. We report, for the first time, evidence that novel viral haplotypes can naturally arise intra-host within an interval as short as two weeks, in approximately 30% of the infected individuals, regardless of symptom severity or immune system deficiencies. Moreover, both phylogenetic and minimum spanning network analyses converge on the hypothesis that the viral sequences evolved from a unique common ancestor haplotype that was carried by an index case. The lockdown extinguished both the viral spread and the emergence of new variants.
Databáze: MEDLINE
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