| Autor: |
Safini N; R&D Virology Laboratory, MCI Santé Animale, Mohammedia 28810, Morocco., Elmejdoub S; R&D Virology Laboratory, MCI Santé Animale, Mohammedia 28810, Morocco., Bamouh Z; R&D Virology Laboratory, MCI Santé Animale, Mohammedia 28810, Morocco., Jazouli M; R&D Virology Laboratory, MCI Santé Animale, Mohammedia 28810, Morocco., Hamdi J; R&D Virology Laboratory, MCI Santé Animale, Mohammedia 28810, Morocco., Boumart Z; R&D Virology Laboratory, MCI Santé Animale, Mohammedia 28810, Morocco., Rhazi H; R&D Virology Laboratory, MCI Santé Animale, Mohammedia 28810, Morocco., Tadlaoui KO; R&D Virology Laboratory, MCI Santé Animale, Mohammedia 28810, Morocco., El Harrak M; R&D Virology Laboratory, MCI Santé Animale, Mohammedia 28810, Morocco. |
| Abstrakt: |
Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP). Lumpy skin disease (LSD) is a viral disease of cattle caused by lumpy skin disease virus (LSDV). LSD and CBPP are both transboundary diseases spreading in the same areas of Africa and Asia. A combination vaccine to control CBPP and LSD offers significant value to small-scale livestock keepers as a single administration. Access to a bivalent vaccine may improve vaccination rates for both pathogens. In the present study, we evaluated the LSDV/CBPP live combined vaccine by testing the generation of virus neutralizing antibodies, immunogenicity, and safety on target species. In-vitro assessment of the Mycoplasma effect on LSDV growth in cell culture was evaluated by infectious virus titration and qPCR during 3 serial passages, whereas in-vivo interference was assessed through the antibody response to vaccination. This combined Mmm/LSDV vaccine could be used to protect cattle against both diseases with a single vaccination in the endemic countries. There were no adverse reactions detected in this study and inoculated cattle produced high levels of specific antibodies starting from day 7 post-vaccination, suggesting that this combination vaccine is both safe and effective. |
