| Autor: |
Xu Z; The New Zealand Institute for Plant and Food Research Limited, Canterbury Agriculture & Science Centre, 74 Gerald Street, Lincoln 7608, New Zealand.; School of Biological Sciences, University of Auckland, Auckland 1142, New Zealand., Khalifa ME; The New Zealand Institute for Plant and Food Research Limited, 120 Mt Albert Road, Sandringham, Auckland 1025, New Zealand., Frampton RA; The New Zealand Institute for Plant and Food Research Limited, Canterbury Agriculture & Science Centre, 74 Gerald Street, Lincoln 7608, New Zealand., Smith GR; The New Zealand Institute for Plant and Food Research Limited, Canterbury Agriculture & Science Centre, 74 Gerald Street, Lincoln 7608, New Zealand., McDougal RL; Scion, New Zealand Forest Research Institute, Ltd., 49 Sala Street, Te Papa Tipu Innovation Park, Rotorua 3010, New Zealand., MacDiarmid RM; School of Biological Sciences, University of Auckland, Auckland 1142, New Zealand.; The New Zealand Institute for Plant and Food Research Limited, 120 Mt Albert Road, Sandringham, Auckland 1025, New Zealand., Kalamorz F; The New Zealand Institute for Plant and Food Research Limited, Canterbury Agriculture & Science Centre, 74 Gerald Street, Lincoln 7608, New Zealand. |
| Abstrakt: |
A new dsRNA virus from the oomycete Phytophthora pluvialis has been characterized and designated as Phytophthora pluvialis RNA virus 1 (PplRV1). The genome of the PplRV1 reference genome is 6742 bp that encodes two predicted open reading frames (ORFs). ORF1 and ORF2 overlap by a 47 nt "slippery" frameshift sequence. ORF1 encodes a putative protein of unknown function. ORF2 shows high similarity to the RNA-dependent RNA polymerase (RdRp) of other dsRNA viruses. Phylogenetic analysis of the putative PplRV1 RdRp and its most closely related viruses showed PplRV1 is distinct from other known viruses (below 33% amino acid similarity), which indicates this virus may belong to a new virus family. Analyses of the geographical distribution of PplRV1 in relation to two genetically distinct classes of its host revealed two corresponding genotypes of the PplRV1 (termed a and b), which share 92.3% nt identity. The reference genome for the second genotype is 6760 bp long and a prediction of its genetic organization shows three ORFs, with ORF2 being split into two ORFs, ORF2a and ORF2b, that is conserved in seven of eleven genotype b isolates. Additionally, a quick and simple diagnostic method using qPCR has been developed, which is suitable for large scale screens to identify PplRV1 in Phytophthora . |
