| Autor: |
Candido TZ; Department of Anesthesiology, Oncology and Radiology, Faculty of Medical Sciences, University of Campinas-UNICAMP, Campinas 13083-887, SP, Brazil., de Paiva REF; Institute of Chemistry, University of Campinas-UNICAMP, Campinas 13083-970, SP, Brazil., Figueiredo MC; Postgraduate Program in Medical Sciences, Faculty of Medical Sciences, University of Campinas-UNICAMP, Campinas 13083-887, SP, Brazil., de Oliveira Coser L; Department of Clinical Pathology, Faculty of Medical Sciences, University of Campinas-UNICAMP, Campinas 13083-887, SP, Brazil., Frajácomo SCL; Department of Biological and Health Sciences, University of Araraquara-UNIARA, Araraquara 14801-320, SP, Brazil., Abbehausen C; Institute of Chemistry, University of Campinas-UNICAMP, Campinas 13083-970, SP, Brazil., Cardinalli IA; Boldrini Children's Center, Department of Pathology, Campinas 13083-210, SP, Brazil., Lustri WR; Department of Biological and Health Sciences, University of Araraquara-UNIARA, Araraquara 14801-320, SP, Brazil., Carvalho JE; Faculty of Pharmaceutical Sciences, University of Campinas-UNICAMP, Campinas 13083-871, SP, Brazil., Ruiz ALTG; Faculty of Pharmaceutical Sciences, University of Campinas-UNICAMP, Campinas 13083-871, SP, Brazil., Corbi PP; Institute of Chemistry, University of Campinas-UNICAMP, Campinas 13083-970, SP, Brazil., Lima CSP; Department of Anesthesiology, Oncology and Radiology, Faculty of Medical Sciences, University of Campinas-UNICAMP, Campinas 13083-887, SP, Brazil. |
| Abstrakt: |
Oxidative stress and inflammation act on skin squamous cell carcinoma (SSCC) development and progression. Curative therapy for SSCC patients is mainly based on surgical resection, which can cause various sequelae. Silver ions have in vitro activities over tumor cells, while nimesulide has antioxidant and anti-inflammatory activities. This study aimed to evaluate the effects of a silver(I) complex with nimesulide (AgNMS) incorporated in a sustained release device based on bacterial cellulose membrane, named AgNMS@BCM, on topic SSCC treatment. The antiproliferative effect of AgNMS complex was evaluated in the SCC4, SCC15 and FaDu SCC lines. AgNMS complex activity on exposure of phosphatidylserine (PS) residues and multicaspase activation were evaluated on FaDu cells by flow cytometry. The AgNMS@BCM effects were evaluated in a SSCC model induced by 7,12-dimethylbenzanthracene/12- o -tetradecanoyl-phorbol-13-acetate (DMBA/TPA) in mice. Toxicity and tumor size were evaluated throughout the study. AgNMS complex showed antiproliferative activity in SCC15 and FaDu lines in low to moderate concentrations (67.3 µM and 107.3 µM, respectively), and induced multicaspase activation on FaDu cells. The AgNMS@BCM did not induce toxicity and reduced tumor size up to 100%. Thus, the application of AgNMS@BCM was effective and safe in SSCC treatment in mice, and can be seen as a potential and safe agent for topic treatment of SSCC in humans. |
