Possible role of nuclear factor erythroid 2-related factor 2 in the progression of human colon precancerous lesions.

Autor: Polimeno L; Polypheno Academic Spin Off, University of Bari 'A. Moro', Policlinico, Piazza G. Cesare 11, 70124 Bari, Italy., Viggiani MT; Gastroenterology Unit, Department of Emergency and Organ Transplantation (DETO), University of Bari 'A. Moro', Policlinico, Piazza G. Cesare 11, 70124 Bari, Italy., Giorgio F; Gastroenterology Unit, Department of Emergency and Organ Transplantation (DETO), University of Bari 'A. Moro', Policlinico, Piazza G. Cesare 11, 70124 Bari, Italy., Polimeno L; Polypheno Academic Spin Off, University of Bari 'A. Moro', Policlinico, Piazza G. Cesare 11, 70124 Bari, Italy., Fratantonio D; Department of Biosciences, Biotechnologies and Biopharmaceutics, Campus Universitario 'G. Quagliarello', University of Bari 'A. Moro', 70125 Bari, Italy., Di Domenico M; Department of Precision Medicine, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Boccellino M; Department of Precision Medicine, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Ballini A; Department of Precision Medicine, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy; School of Medicine, University of Bari Aldo Moro, 70124 Bari, Italy., Topi S; Department of Clinical Disciplines, School of Technical Medical Sciences, University of Elbasan 'A. Xhuvani', Rruga Ismail Zyma, 3001 Elbasan, Albania., Di Leo A; Gastroenterology Unit, Department of Emergency and Organ Transplantation (DETO), University of Bari 'A. Moro', Policlinico, Piazza G. Cesare 11, 70124 Bari, Italy., Santacroce L; Polypheno Academic Spin Off, University of Bari 'A. Moro', Policlinico, Piazza G. Cesare 11, 70124 Bari, Italy; Department of Clinical Disciplines, School of Technical Medical Sciences, University of Elbasan 'A. Xhuvani', Rruga Ismail Zyma, 3001 Elbasan, Albania; Department of Interdisciplinary Medicine, Microbiology and Virology Unit, Policlinico University Hospital of Bari, University of Bari Aldo Moro, 70124 Bari, Italy., Barone M; Gastroenterology Unit, Department of Emergency and Organ Transplantation (DETO), University of Bari 'A. Moro', Policlinico, Piazza G. Cesare 11, 70124 Bari, Italy. Electronic address: michele.barone@uniba.it.
Jazyk: angličtina
Zdroj: Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver [Dig Liver Dis] 2022 Dec; Vol. 54 (12), pp. 1716-1720. Date of Electronic Publication: 2022 Feb 21.
DOI: 10.1016/j.dld.2022.01.131
Abstrakt: Background: Increased levels of oxidative stress/cell inflammation contribute to colorectal cancer (CRC) onset. Nuclear factor-erythroid 2-related factor 2 (Nrf2) and its controlled growth factor erv1-like (Gfer) gene regulate redox-sensitive and anti-inflammatory mechanisms, respectively, which can contribute to promoting cancer development.
Aim: We evaluated Nrf2 and Gfer RNA expression and Nrf2 protein expression in colon mucosa in order to establish their possible involvement in the early stage of CRC.
Methods: Forty subjects were enrolled after a histological evaluation of their colon biopsies. They included 20 subjects with a sporadic colorectal adenoma (SpCA group) and 20 without precancerous lesions (controls). Biopsy samples were processed for gene expression analysis and protein expression, using Real-time PCR and immunofluorescence confocal microscopy, respectively.
Results: Nrf2 and Gfer mRNA expression were significantly reduced (p=0.007 and p<0.003, respectively) in SpCA tissues compared to normal mucosa from controls. Furthermore, immunofluorescence analysis confirmed a relevant reduction of Nrf2 in SpCA tissue compared to normal tissue from controls.
Conclusions: Our data confirm the hypothesis that Nrf2 and Gfer expression may be involved in the initial hits contributing to the multistep process of colon carcinogenesis. Further larger studies are needed to confirm if Nrf2 and Gfer are potential risk/prognostic factors for cancer development.
Competing Interests: Declaration of Competing Interest None declared.
(Copyright © 2022 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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