| Autor: |
Díaz Calvo T; Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK., Tejera N; Microbes in the Food Chain, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK., McNamara I; Norwich Medical School, University of East Anglia, Norwich NR4 7UQ, UK.; Department of Orthopaedics and Trauma, Norfolk and Norwich University Hospital NHS Foundation Trust, Norwich NR4 7UY, UK., Langridge GC; Microbes in the Food Chain, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK., Wain J; Microbes in the Food Chain, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK.; Norwich Medical School, University of East Anglia, Norwich NR4 7UQ, UK., Poolman M; Cell System Modelling Group, Oxford Brookes University, Oxford OX3 OBP, UK., Singh D; Microbes in the Food Chain, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK. |
| Abstrakt: |
Staphylococcus epidermidis is a common commensal of collagen-rich regions of the body, such as the skin, but also represents a threat to patients with medical implants (joints and heart), and to preterm babies. Far less studied than Staphylococcus aureus , the mechanisms behind this increasingly recognised pathogenicity are yet to be fully understood. Improving our knowledge of the metabolic processes that allow S. epidermidis to colonise different body sites is key to defining its pathogenic potential. Thus, we have constructed a fully curated, genome-scale metabolic model for S. epidermidis RP62A, and investigated its metabolic properties with a focus on substrate auxotrophies and its utilisation for energy and biomass production. Our results show that, although glucose is available in the medium, only a small portion of it enters the glycolytic pathways, whils most is utilised for the production of biofilm, storage and the structural components of biomass. Amino acids, proline, valine, alanine, glutamate and arginine, are preferred sources of energy and biomass production. In contrast to previous studies, we have shown that this strain has no real substrate auxotrophies, although removal of proline from the media has the highest impact on the model and the experimental growth characteristics. Further study is needed to determine the significance of proline, an abundant amino acid in collagen, in S. epidermidis colonisation. |
