| Autor: |
Gheno E; Post-Graduation Program in Dentistry, Fluminense Federal University, Niteroi 24220-140, RJ, Brazil.; Surgical Sciences and Integrated Diagnostics Department, University of Genoa, 16132 Genoa, Italy., Alves GG; Cell and Molecular Biology Department, Institute of Biology, Fluminense Federal University, Niteroi 24220-000, RJ, Brazil.; Clinical Research Unit of the Antonio Pedro Hospital, Fluminense Federal University, Niteroi 24220-000, RJ, Brazil., Ghiretti R; Maxillofacial Surgeon, Private Practitioner, 46047 Porto, Italy., Mello-Machado RC; Post-Graduation Program in Dentistry, Fluminense Federal University, Niteroi 24220-140, RJ, Brazil.; Implant Dentistry Department, Universidade Iguaçu, Nova Iguaçu 26260-045, RJ, Brazil., Signore A; Surgical Sciences and Integrated Diagnostics Department, University of Genoa, 16132 Genoa, Italy.; Therapeutic Dentistry Department, Institute of Dentistry, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia., Lourenço ES; Post-Graduation Program in Dentistry, Fluminense Federal University, Niteroi 24220-140, RJ, Brazil.; Clinical Research Unit of the Antonio Pedro Hospital, Fluminense Federal University, Niteroi 24220-000, RJ, Brazil., Leite PEC; Post-Graduation Program in Sciences and Biotechnology, Fluminense Federal University, Niteroi 24220-000, RJ, Brazil., Mourão CFAB; Clinical Research Unit of the Antonio Pedro Hospital, Fluminense Federal University, Niteroi 24220-000, RJ, Brazil., Sohn DS; Department of Dentistry and Oral and Maxillofacial Surgery, Catholic University Medical Center of Daegu, Daegu 705-718, Korea., Calasans-Maia MD; Department of Oral Surgery, Dentistry School, Fluminense Federal University, Niteroi 24020-140, RJ, Brazil. |
| Abstrakt: |
Sticky bone, a growth factor-enriched bone graft matrix, is a promising autologous material for bone tissue regeneration. However, its production is strongly dependent on manual handling steps. In this sense, a new device was developed to simplify the confection of the sticky bone, named Sticky Bone Preparation Device (SBPD ® ). The purpose of this pilot study was to investigate the suitability of the SBPD ® to prepare biomaterials for bone regeneration with autologous platelet concentrates. The SBPD ® allows the blending of particulate samples from synthetic, xenograft, or autogenous bone with autologous platelet concentrates, making it easy to use and avoiding the need of further manipulations for the combination of the materials. The protocol for the preparation of sticky bone samples using the SBPD ® is described, and the resulting product is compared with hand-mixed SB preparations regarding in vitro parameters such as cell content and the ability to release growth factors and cytokines relevant to tissue regeneration. The entrapped cell content was estimated, and the ability to release biological mediators was assessed after 7 days of incubation in culture medium. Both preparations increased the leukocyte and platelet concentrations compared to whole-blood samples ( p < 0.05), without significant differences between SB and SBPD ® . SBPD ® samples released several growth factors, including VEGF, FGFb, and PDGF, at concentrations physiologically equivalent to those released by SB preparations. Therefore, the use of SBPD ® results in a similar product to the standard protocol, but with more straightforward and shorter preparation times and less manipulation. These preliminary results suggest this device as a suitable alternative for combining bone substitute materials with platelet concentrates for bone tissue regeneration. |
