| Autor: |
Akita D; Department of Partial Denture Prosthodontics, Nihon University School of Dentistry, Tokyo 101-8310, Japan., Kazama T; Department of Functional Morphology, Division of Cell Regeneration and Transplantation, Nihon University School of Medicine, Tokyo 173-8601, Japan., Tsukimura N; Department of Partial Denture Prosthodontics, Nihon University School of Dentistry, Tokyo 101-8310, Japan., Taniguchi Y; Section of Laboratory Animals, Nihon University School of Medicine, Tokyo 173-8601, Japan., Takahashi R; Section of Laboratory Animals, Nihon University School of Medicine, Tokyo 173-8601, Japan., Arai Y; Department of Oral and Maxillofacial Radiology, Nihon University School of Dentistry, Tokyo 101-8310, Japan., Tsurumachi-Iwasaki N; Department of Orthodontics, Nihon University School of Dentistry, Tokyo 101-8310, Japan., Yasuda H; Department of Partial Denture Prosthodontics, Nihon University School of Dentistry, Tokyo 101-8310, Japan., Okubo T; Department of Partial Denture Prosthodontics, Nihon University School of Dentistry, Tokyo 101-8310, Japan., Kano K; Laboratory of Cell and Tissue Biology, College of Bioresource Science, Nihon University, Fujisawa 252-0880, Japan., Matsumoto T; Department of Functional Morphology, Division of Cell Regeneration and Transplantation, Nihon University School of Medicine, Tokyo 173-8601, Japan., Honda M; Department of Oral Anatomy, Aichi Gakuin University School of Dentistry, Nagoya 464-8650, Japan. |
| Abstrakt: |
Adipose tissue is composed mostly of adipocytes that are in contact with capillaries. By using a ceiling culture method based on buoyancy, lipid-free fibroblast-like cells, also known as dedifferentiated fat (DFAT) cells, can be separated from mature adipocytes with a large single lipid droplet. DFAT cells can re-establish their active proliferation ability and transdifferentiate into various cell types under appropriate culture conditions. Herein, we sought to compare the regenerative potential of collagen matrix alone (control) with autologous DFAT cell-loaded collagen matrix transplantation in adult miniature pigs (microminipigs; MMPs). We established and transplanted DFAT cells into inflammation-inducing periodontal class II furcation defects. At 12 weeks after cell transplantation, a marked attachment gain was observed based on the clinical parameters of probing depth (PD) and clinical attachment level (CAL). Additionally, micro computed tomography (CT) revealed hard tissue formation in furcation defects of the second premolar. The cemento-enamel junction and alveolar bone crest distance was significantly shorter following transplantation. Moreover, newly formed cellular cementum, well-oriented periodontal ligament-like fibers, and alveolar bone formation were observed via histological analysis. No teratomas were found in the internal organs of recipient MMPs. Taken together, these findings suggest that DFAT cells can safely enhance periodontal tissue regeneration. |
