| Autor: |
Kotlova ER; Komarov Botanical Institute, Russian Academy of Sciences RAS, 197376 Saint-Petersburg, Russia., Senik SV; Komarov Botanical Institute, Russian Academy of Sciences RAS, 197376 Saint-Petersburg, Russia., Manzhieva BS; Komarov Botanical Institute, Russian Academy of Sciences RAS, 197376 Saint-Petersburg, Russia., Kiyashko AA; Komarov Botanical Institute, Russian Academy of Sciences RAS, 197376 Saint-Petersburg, Russia., Shakhova NV; Komarov Botanical Institute, Russian Academy of Sciences RAS, 197376 Saint-Petersburg, Russia., Puzansky RK; Komarov Botanical Institute, Russian Academy of Sciences RAS, 197376 Saint-Petersburg, Russia., Volobuev SV; Komarov Botanical Institute, Russian Academy of Sciences RAS, 197376 Saint-Petersburg, Russia., Misharev AD; Chemical Analysis and Materials Research Centre, Saint-Petersburg State University, 198504 Saint-Petersburg, Russia., Serebryakov EB; Chemical Analysis and Materials Research Centre, Saint-Petersburg State University, 198504 Saint-Petersburg, Russia., Psurtseva NV; Komarov Botanical Institute, Russian Academy of Sciences RAS, 197376 Saint-Petersburg, Russia. |
| Abstrakt: |
Phosphatidylcholines (PC) are the main membrane lipid constituents comprising more than 50% of total glycerophospholipids. They coordinate a number of cell functions, particularly cell growth, homeostasis, secretion, recognition and communication. In basidial fungi PC are synthesized via the Kennedy pathway as well as through methylation of phosphatidylethanolamines (PE) and then undergo remodeling in Lands cycle that replaces fatty acids in PC molecules. The molecular profile of PC is determined by the genetic features that are characteristic for every species and depend on the environment. Here we present the results of ESI-MS based analyses of PC profiles of 38 species of basidiomycetes belonging to Agaricales (12), Polyporales (17), Russulales (5), Gleophyllales (2), Cantharellales (1), Auriculariales (1), Phallales (1). Although the variety of PC molecular species of basidiomycetes is rather diverse (20-38 molecular species in every profile), only 1-3 main molecular species represent 70-90% of total PC content. The most abundant of them are C36:4 and C36:3, followed by C34:1, C34:2, C36:5, C36:2. In the majority of basidiomycetes, C36:4 reaches up to 50-70% of total PC molecular species. Based on the results of hierarchical cluster analysis four main types of PC profiles which characterized the studied fungi independently from their taxonomic position, ecology, trophic status, and hyphal differentiation have been revealed. Comparative analyses of studied fungi using PCA method have shown that species of Polyporales differ from those of Agaricales by higher variability of PC profiles. |
