| Autor: |
Hernández-Aguirre LE; Molecular Biology Laboratory, Chemical Sciences Faculty, Circuit # 1 New Universitarium Campus, Autonomous University of Chihuahua (UACH), Chihuahua 31125, Chihuahua, Mexico., Fuentes-Sidas YI; Molecular Biology Laboratory, Chemical Sciences Faculty, Circuit # 1 New Universitarium Campus, Autonomous University of Chihuahua (UACH), Chihuahua 31125, Chihuahua, Mexico., Rivera-Rangel LR; Molecular Biology Laboratory, Chemical Sciences Faculty, Circuit # 1 New Universitarium Campus, Autonomous University of Chihuahua (UACH), Chihuahua 31125, Chihuahua, Mexico., Gutiérrez-Méndez N; Molecular Biology Laboratory, Chemical Sciences Faculty, Circuit # 1 New Universitarium Campus, Autonomous University of Chihuahua (UACH), Chihuahua 31125, Chihuahua, Mexico., Yepiz-Plascencia G; Research Center in Food & Development (CIAD), Gustavo Enrique Astiazarán Rosas Road, No 46, La Victoria Suburb, Hermosillo 83304, Sonora, Mexico., Chávez-Flores D; Molecular Biology Laboratory, Chemical Sciences Faculty, Circuit # 1 New Universitarium Campus, Autonomous University of Chihuahua (UACH), Chihuahua 31125, Chihuahua, Mexico., Zavala-Díaz de la Serna FJ; Molecular Biology Laboratory, Chemical Sciences Faculty, Circuit # 1 New Universitarium Campus, Autonomous University of Chihuahua (UACH), Chihuahua 31125, Chihuahua, Mexico., Peralta-Pérez MDR; Molecular Biology Laboratory, Chemical Sciences Faculty, Circuit # 1 New Universitarium Campus, Autonomous University of Chihuahua (UACH), Chihuahua 31125, Chihuahua, Mexico., García-Triana A; Molecular Biology Laboratory, Chemical Sciences Faculty, Circuit # 1 New Universitarium Campus, Autonomous University of Chihuahua (UACH), Chihuahua 31125, Chihuahua, Mexico. |
| Abstrakt: |
Glutathione peroxidase 3 (GPx3) is the only extracellular selenoprotein (Sel) that enzymatically reduces H 2 O 2 to H 2 O and O 2 . Two GPx3 ( CqGPx3 ) cDNAs were characterized from crayfish Cherax quadricarinatus . The nerve cord CqGPx3a isoform encodes for a preprotein containing an N-terminal signal peptide of 32 amino acid residues, with the mature Sel region of 192 residues and a dispensable phosphorylation domain of 36 residues. In contrast, the pereiopods CqGPx3b codes for a precursor protein with 19 residues in the N-terminal signal peptide, then the mature 184 amino acid residues protein and finally a Pro-rich peptide of 42 residues. CqGPx3 are expressed in cerebral ganglia, pereiopods and nerve cord. CqGPx3a is expressed mainly in cerebral ganglia, antennulae and nerve cord, while CqGPx3b was detected mainly in pereiopods. CqGPx3a expression increases with high temperature and hypoxia; meanwhile, CqGPx3b is not affected. We report the presence and differential expression of GPx3 isoforms in crustacean tissues in normal conditions and under stress for high temperature and hypoxia. The two isoforms are tissue specific and condition specific, which could indicate an important role of CqGPx3a in the central nervous system and CqGPx3b in exposed tissues, both involved in different responses to environmental stressors. |
