Modulation of the cholinergic pathway induced by skin secretion of Phyllomedusa iheringii Boulenger, 1885 in a vertebrate model.

Autor: Borges BT; Laboratório de Neurobiologia e Toxinologia, Universidade Federal do Pampa, São Gabriel, RS, Brazil. Electronic address: brunatrborges@outlook.com., Oliveira RS; Laboratório de Neurobiologia e Toxinologia, Universidade Federal do Pampa, São Gabriel, RS, Brazil., Leal AP; Laboratório de Neurobiologia e Toxinologia, Universidade Federal do Pampa, São Gabriel, RS, Brazil; Programa de Pós-Graduação em Ciências Biológicas e Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Rosa ME; Laboratório de Neurobiologia e Toxinologia, Universidade Federal do Pampa, São Gabriel, RS, Brazil., Barreto YC; Laboratório de Neurobiologia e Toxinologia, Universidade Federal do Pampa, São Gabriel, RS, Brazil., Gomes Dos Santos T; Laboratório de Estudos em Biodiversidade Pampiana, Universidade Federal do Pampa, São Gabriel, RS, Brazil., de Brum Vieira P; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa, São Gabriel, RS, Brazil., Dal Belo CA; Laboratório de Neurobiologia e Toxinologia, Universidade Federal do Pampa, São Gabriel, RS, Brazil; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa, São Gabriel, RS, Brazil., Vinadé L; Laboratório de Neurobiologia e Toxinologia, Universidade Federal do Pampa, São Gabriel, RS, Brazil; Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa, São Gabriel, RS, Brazil.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2022 Apr 30; Vol. 210, pp. 115-122. Date of Electronic Publication: 2022 Feb 22.
DOI: 10.1016/j.toxicon.2022.02.017
Abstrakt: Amphibians represent one of the main natural sources of bioactive molecules of interest to biotechnological research. The Phyllomedusidae family has several species occurring in Brazil and some studies demonstrate the biological potential of poisons of these species, however many still need to be characterized. Phyllomedusa iheringii is endemic in Brazilian and Uruguayan Pampa Biome and has little data in the literature regarding the action of its poison on experimental organisms. Thus, the present work evaluates the biological activity of P. iheringii secretion on the central and peripheral nervous system of a vertebrate model. The skin secretions of P. iheringii (SSPI) were collected through manual compression and electrical stimulation of the animal's bodies. The resulting content was used in neurobiological tests searching for modulatory effects on the main pathways involved in the neurotoxicity mechanism of vertebrates. SSPI affected the contraction force of the chick biventer cervicis muscle (Gallus gallus domesticus) at some concentrations used (5, 10, and 12 μg/mL). In slices from the cerebral cortex of G. gallus domesticus an increase in cell viability was observed after treatment with SSPI (10 μg/mL) and a neuroprotective effect when treated simultaneously with hydrogen peroxide (H 2 O 2 ), Neostigmine (NEO) and Trichlorfon (TRI). The cholinergic pathway is possibly the main pathway modulated by SSPI since assays with the cerebral cortex and biventer cervicis muscle demonstrated the increased activity of the enzyme acetylcholinesterase (AChE) (SSPI 10 μg/mL and 12 μg/mL, respectively). SSPI (10 μg/mL) also prevented the modulation of NEO and TRI, two recognized anticholinesterase agents, in AChE activity in slices of the cerebral cortex. Therefore, our results have demonstrated the unpublished biotechnological potential of P. iheringii over the vertebrate model and its modulation on the nervous system, with apparent action on the cholinergic pathway.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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