Hygiene Indicators and Salmonellae on Surfaces of Swine Carcasses from Two Slaughterhouses in Northern Portugal.

Autor:	Moura-Alves M; Veterinary and Animal Research Centre (CECAV), Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal., Carvalho M; Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences (ECAV), Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal., Baggio Ribeiro DH; Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences (ECAV), Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal.; Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal., Barbosa J; Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences (ECAV), Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal., Silveira L; Department of Food Science and Technology, School of Agricultural Sciences, Federal University of Santa Catarina, Rod. Ademar Gonzaga 1346, 88034-001 Florianópolis, Santa Catarina, Brazil., Pista Â; Department of Food Science and Technology, School of Agricultural Sciences, Federal University of Santa Catarina, Rod. Ademar Gonzaga 1346, 88034-001 Florianópolis, Santa Catarina, Brazil., Pinto HP; National Reference Laboratory of Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health, Avenida Padre Cruz, 1649-016 Lisboa, Portugal., Saraiva C; Veterinary and Animal Research Centre (CECAV), Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal.; National Reference Laboratory of Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health, Avenida Padre Cruz, 1649-016 Lisboa, Portugal., Teixeira P; Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences (ECAV), Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal., Esteves A; Veterinary and Animal Research Centre (CECAV), Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal.; National Reference Laboratory of Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health, Avenida Padre Cruz, 1649-016 Lisboa, Portugal.
Jazyk:	angličtina
Zdroj:	Journal of food protection [J Food Prot] 2022 Nov 01; Vol. 85 (11), pp. 1566-1575.
DOI:	10.4315/JFP-21-312
Abstrakt:	Abstract: The monitoring of carcass surface contamination along the slaughter line enables verification of slaughter operation hygiene and the use of good manufacturing practices. Pork meat is a common source of human nontyphoidal salmonellosis, one of the most frequently reported foodborne illnesses worldwide. This study was conducted to gather data on microbial loads before and after evisceration on the surfaces of swine carcasses in two slaughterhouses. The presence of Salmonella enterica was evaluated only after evisceration on carcass surfaces and in livers and floor drains (environmental samples) because pigs are common carriers of this pathogen. The contamination of carcass surfaces was evaluated by delimitation of surface area with sterilized templates (100 cm2), and surface samples were collected with gauze swabs. Total aerobic mesophilic bacteria, Enterobacteriaceae, and Escherichia coli were enumerated. Significant differences (P < 0.05) in counts of mesophilic bacteria, Enterobacteriaceae, and E. coli were found on the external carcass surfaces, with higher counts after evisceration. The neck and abdominal areas had higher levels of mesophilic bacteria, Enterobacteriaceae, and E. coli and a high prevalence of Salmonella. Salmonella was detected in only one of the studied slaughterhouses; 19 (7.3%) of 259 analyzed carcass samples were positive for Salmonella, and Salmonella was detected in two livers and two floor drains. A total of 52 Salmonella isolates (44 from carcasses, 5 from livers, and 3 from drains) were recovered. Three Salmonella serovars (Typhimurium 4,5:i- , Wernigerone, and Derby) were identified, and 53.8% of the 52 isolates were multidrug resistant. The results reveal the need for continuous improvement of slaughtering operations and implementation of good manufacturing practices to ensure the safety of pork produced in Portugal. (Copyright ©, International Association for Food Protection.)
Databáze:	MEDLINE
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