Microalgae-Based Fluorimetric Bioassays for Studying Interferences on Photosynthesis Induced by Environmentally Relevant Concentrations of the Herbicide Diuron.

Autor:	Grasso G; Istituto per lo Studio dei Materiali Nanostrutturati Sede Sapienza, Consiglio Nazionale delle Ricerche, P. le Aldo Moro 5, 00185 Rome, Italy., Cocco G; Dipartimento di Scienze e Tecnologie per l'Agricoltura, le Foreste, la Natura e l'Energia, Università degli Studi della Tuscia, 01100 Viterbo, Italy., Zane D; Istituto per lo Studio dei Materiali Nanostrutturati Sede Sapienza, Consiglio Nazionale delle Ricerche, P. le Aldo Moro 5, 00185 Rome, Italy., Frazzoli C; Dipartimento Malattie Cardiovascolari e Endocrino-metaboliche, e Invecchiamento, Istituto Superiore di Sanità, Via Giano della Bella, 34, 00162 Rome, Italy., Dragone R; Istituto per lo Studio dei Materiali Nanostrutturati Sede Sapienza, Consiglio Nazionale delle Ricerche, P. le Aldo Moro 5, 00185 Rome, Italy.
Jazyk:	angličtina
Zdroj:	Biosensors [Biosensors (Basel)] 2022 Jan 25; Vol. 12 (2). Date of Electronic Publication: 2022 Jan 25.
DOI:	10.3390/bios12020067
Abstrakt:	The widespread agricultural use of the phenylurea herbicide Diuron (DCMU) requires the investigation of ecotoxicological risk in freshwater and soil ecosystems in light of potential effects on non-target primary producers and a heavier effect on higher trophic levels. We used microalgae-based fluorimetric bioassays for studying the interferences on the photosynthesis of a freshwater and soil model green microalga ( Chlamydomonas reinhardtii ) induced by environmentally relevant concentrations of the herbicide DCMU. Measurements of steady-state chlorophyll a (Chl-a) fluorescence emission spectra were performed; as well, the kinetics of the Chl-a fluorescence transient were recorded. Percentage indexes of interference on photosynthesis were calculated after comparison of steady-state and kinetic Chl-a fluorescence measurements of DCMU-exposed and control C. reinhardtii cell suspensions. The results obtained after 30 min exposure to the herbicide DCMU confirmed a significant inhibitory effect of DCMU 2 μg/L, and no significant differences between %ι values for DCMU 0.2 μg/L and 0.02 μg/L exposures. Positive %ε values from kinetic measurements of the Chl-a fluorescence transient confirmed the same interfering effect of 2 μg/L DCMU on PSII photochemistry in the exposed C. reinhardtii cell suspensions. Negative values of %ε observed for 0.2 and 0.02 μg/L DCMU exposures could be attributable to a presumptive 'stimulatory-like' effect in the photochemistry of photosynthesis. Short-term exposure to sub-μg/L DCMU concentration (≤0.2 μg/L) affects the photosynthetic process of the model microalga C. reinhardtii . Similar environmental exposures could affect natural communities of unicellular autotrophs, with hardly predictable cascading secondary effects on higher trophic levels.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu