Fake news blues: A GUS staining protocol to reduce false-negative data.

Autor: Dedow LK; Department of Molecular, Cell and Developmental Biology University of California Los Angeles Los Angeles CA USA., Oren E; Department of Molecular, Cell and Developmental Biology University of California Los Angeles Los Angeles CA USA., Braybrook SA; Department of Molecular, Cell and Developmental Biology University of California Los Angeles Los Angeles CA USA.
Jazyk: angličtina
Zdroj: Plant direct [Plant Direct] 2022 Feb 14; Vol. 6 (2), pp. e367. Date of Electronic Publication: 2022 Feb 14 (Print Publication: 2022).
DOI: 10.1002/pld3.367
Abstrakt: The β-glucuronidase gene, uidA ( GUS ), has remained a favorite reporter gene in plants since its introduction in 1987 for its stability and versatility in a variety of fluorometric, spectrophotometric, and histochemical techniques. One of the most popular uses is as a reporter gene for visualizing endogenous promoter activities within plant tissues. Despite this popularity, specific protocols for minimizing nonrepresentative staining patterns, including false negatives, in challenging tissue types are not common. This became a large issue during our work on dark-grown Arabidopsis hypocotyls, and we set out to develop a protocol that would ensure accurate staining in a tissue that is biologically resistant to reagent penetration. Through extensive testing using a variety of constitutive and endogenous promoter::GUS fusion lines, we have developed an optimized GUS staining protocol that combines the use of acetone as a fixative, deliberate physical damage, and proper positive and negative controls to help ensure accurate staining along the hypocotyl while minimizing false negatives. Hopefully, our recommendations will allow for improved staining that more accurately reflects the true activity of cloned endogenous promoters and thus facilitate a more accurate understanding of promoter activity in Arabidopsis hypocotyls and other hard-to-stain tissues.
Competing Interests: The authors did not report any conflict of interest.
(© 2022 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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